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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2017 / Cilt: 41 - Sayı: 1

Molecular cloning and sequence characterization of two genes, DQA1 and DQA2, from the Chinese yakow ( Bos grunniens × Bos taurus )

Dongmei XI
Sameeullah MEMON
Guozhi LI
Xiangying LIU
Chao SU
Jing LENG
Liping WANG
Sehroon KHAN
Weidong DENG
Sayfa
136–141
Yayın Tarihi
2017-02-21
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

This result will deepen our understanding of MHC diversity among different ruminant species based on comparative population genetics.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:The major histocompatibility complex (MHC) plays a crucial role in the immune response to infectious diseases.
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Temel Bulgular & Veri:Two full-length cDNA sequences and partial 5'- and 3'-untranslated regions (UTRs) were retrieved and denoted as Bogr×BoLA-DQA*0101 (DQA1) and BogrxBoLA-DQA*2001 (DQA2), respectively.
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Sonuç & Katkı:This result will deepen our understanding of MHC diversity among different ruminant species based on comparative population genetics.
🔬Tür:Araştırma Makalesi
📍Kapsam:2001 Verileri
⚙️Değişkenler:Major histocompatibility complex (MHC) · Chinese yakow ( Bos grunniens × Bos taurus ) · DQA1 and DQA2 genes

Abstract

The major histocompatibility complex (MHC) plays a crucial role in the immune response to infectious diseases. The aim of the present study was to investigate the structural and functional characteristics and possible duplication of the MHC-DQA genes in the Chinese yakow, which is well adapted to the Qinghai?Tibetan Plateau. Two full-length cDNA sequences and partial 5'- and 3'-untranslated regions (UTRs) were retrieved and denoted as Bogr×BoLA-DQA*0101 (DQA1) and BogrxBoLA-DQA*2001 (DQA2), respectively. The percentage of nucleotide and amino acid sequences homology between BogrxBoLA-DQA1 and BogrxBoLA-DQA2 showed that these two sequences have more identity to alleles of the corresponding DQA1 and DQA2 genes from other counterparts than to each other. Moreover, the Bogr×BoLA-DQA1 and -DQA2 exhibited considerable variation with 99 nucleotide polymorphisms along the length of the coding regions, leading to 52 amino acid polymorphisms. The phylogenetic tree investigation also demonstrated that there is a larger genetic gap within these two identified genes than within orthologous genes from different animals, suggesting that these sequences belong to nonallelic and duplicated types in the Chinese yakow. This is the first report to isolate and identify the MHC-DQA1 and -DQA2 genes. This result will deepen our understanding of MHC diversity among different ruminant species based on comparative population genetics.

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Bu Makaleye Atıf Yap

XI, D., MEMON, S., LI, G., LIU, X., SU, C., LENG, J., WANG, L., KHAN, S., DENG, W. (2017). Molecular cloning and sequence characterization of two genes, DQA1 and DQA2, from the Chinese yakow ( Bos grunniens × Bos taurus ). Turkish Journal of Veterinary and Animal Sciences, Cilt: 41 - Sayı: 1, 136–141. https://ajindex.com/articles/121513

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Kaynakça (15)

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