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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2014 / Cilt: 38 - Sayı: 5

Impact of the depletion of high-abundance proteins in blood plasma/serum on the proteome of these media in growing farm animals

Adam LEPCZYNSKI
Alicja DRATWA-CHALUPNIK
Agnieszka HEROSIMCZYK
Sayfa
490–495
Yayın Tarihi
2014-05-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Increased content of the low-abundance proteins on the 2D gels enables complete analysis of plasma/serum proteome and thereby allows for the discovery of novel biomarkers that might be useful in veterinary medicine.

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Amaç & Hipotez:The aim of the study was to use hexapeptide combinatorial libraries to evaluate the influence of high-abundance protein depletion on low-abundance protein enrichment, protein concentration, and 2D gel quality in plasma and serum proteomes of growing piglets and calves.
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Temel Bulgular & Veri:Depleted and nondepleted samples of plasma/serum were resolved using 2D electrophoresis (2DE).
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Sonuç & Katkı:Increased content of the low-abundance proteins on the 2D gels enables complete analysis of plasma/serum proteome and thereby allows for the discovery of novel biomarkers that might be useful in veterinary medicine.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Proteome · high-abundance protein depletion · 2-dimensional electrophoresis

Özet

The aim of the study was to use hexapeptide combinatorial libraries to evaluate the influence of high-abundance protein depletion on low-abundance protein enrichment, protein concentration, and 2D gel quality in plasma and serum proteomes of growing piglets and calves. Depleted and nondepleted samples of plasma/serum were resolved using 2D electrophoresis (2DE). Bioinformatic analysis of the 2D gels showed an increased number of detected spots on each gel after depletion of excess high-abundance proteins in calf plasma (+26.46%) and in piglet serum (+49.40%). Relative expression of selected low-abundance protein spots in the blood plasma/serum samples was higher after high-abundance protein depletion. Both media showed decreased expressions of high-abundance protein spot clusters on 2D gels after treatment. The results of this study confirm both the high efficiency of high-abundance protein depletion and the increase in concentrations of low-abundance proteins on 2D gels after treatment. The method of depletion is reproducible and it may be used as an alternative to immunodepletion for preparing blood plasma/serum samples from young, growing animals for 2DE. Increased content of the low-abundance proteins on the 2D gels enables complete analysis of plasma/serum proteome and thereby allows for the discovery of novel biomarkers that might be useful in veterinary medicine.

Abstract

The aim of the study was to use hexapeptide combinatorial libraries to evaluate the influence of high-abundance protein depletion on low-abundance protein enrichment, protein concentration, and 2D gel quality in plasma and serum proteomes of growing piglets and calves. Depleted and nondepleted samples of plasma/serum were resolved using 2D electrophoresis (2DE). Bioinformatic analysis of the 2D gels showed an increased number of detected spots on each gel after depletion of excess high-abundance proteins in calf plasma (+26.46%) and in piglet serum (+49.40%). Relative expression of selected low-abundance protein spots in the blood plasma/serum samples was higher after high-abundance protein depletion. Both media showed decreased expressions of high-abundance protein spot clusters on 2D gels after treatment. The results of this study confirm both the high efficiency of high-abundance protein depletion and the increase in concentrations of low-abundance proteins on 2D gels after treatment. The method of depletion is reproducible and it may be used as an alternative to immunodepletion for preparing blood plasma/serum samples from young, growing animals for 2DE. Increased content of the low-abundance proteins on the 2D gels enables complete analysis of plasma/serum proteome and thereby allows for the discovery of novel biomarkers that might be useful in veterinary medicine.

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Bu Makaleye Atıf Yap

LEPCZYNSKI, A., DRATWA-CHALUPNIK, A., HEROSIMCZYK, A. (2014). Impact of the depletion of high-abundance proteins in blood plasma/serum on the proteome of these media in growing farm animals. Turkish Journal of Veterinary and Animal Sciences, Cilt: 38 - Sayı: 5, 490–495. https://ajindex.com/articles/121479

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Kaynakça (10)

  1. Miller I, Wait R, Sipos W, Gemeiner M. A proteomic reference map for pig serum proteins as a prerequisite for diagnostic applications. Res Vet Sci 2009; 86: 362–367. 2. Hu S, Loo JA, Wong DT. Human body fluid proteome analysis. Proteomics 2006; 6: 6326–6353.
  2. Karczmarski J, Mikula M, Henning E, Rubel T, Otrowski J. Perspectives of mass spectrometry-based disease biomarkers discovery in plasma or serum peptidome. Postępy Nauk Medycznych 2009; 2: 149–156 (article in Polish with English abstract). Herosimczyk A, Dejeans N, Sayd T, Ożgo M, Skrzypczak WF, Mazur A. Plasma proteome analysis: 2D gels and chips. J Physiol Pharmacol 2006; 57: 81–93.
  3. Bendixen E, Danielsen M, Hollung K, Gianazza E, Miller I. Farm animal proteomics—a review. J Proteomics 2011; 74: 282–293. 6. D’Ambrosio C, Arena S, Talamo F, Ledda L, Renzone G, Ferrara L, Scaloni A. Comparative proteomic analysis of mammalian animal tissues and body fluids: bovine proteome database. J Chromatogr B 2005; 815: 157–168.
  4. Huang Y, Olson DJ, Gordon JR, Middleton DM, Simko E. Plasma protein profiles of neonatal pigs before and after suckling. Can J Vet Res 2012; 76: 1–7.
  5. Skrzypczak WF, Ożgo M, Herosimczyk A, Lepczyński A. Defining the blood plasma protein repertoire of seven day old dairy calves - a preliminary study. J Physiol Pharmacol 2011; 62: 313–319.
  6. Wait R, Miller I, Eberini I, Cairoli F, Veronesi C, Battocchio M, Gemeiner M, Gianazza E. Strategies for proteomic with incompletely characterized genomes: the proteome of Bos taurus serum. Electrophoresis 2002; 23: 3418–3427.
  7. Herosimczyk A, Lepczynski A, Ozgo M, Dratwa-Chałupnik A, Michalek K, Skrzypczak WF. Blood plasma protein and lipid profile changes in calves during the first week of life. Pol J Vet Sci 2013; 16: 425–434.
  8. Xia C, Zhang HY, Wu L, Xu C, Zheng JS, Yan YJ, Yang LJ, Shu S. Proteomic analysis of plasma from cows affected with milk fever using two-dimensional differential in-gel electrophoresis and mass spectrometry. Res Vet Sci 2012; 93: 857–861.
  9. Chen YY, Lin SY, Yeh YY, Hsiao HH, Wu CY, Chen ST, Wang AHJ. A modified protein precipitation procedure for efficient removal of albumin from serum. Electrophoresis 2005; 26: 2117–2127. Chevalier F. Highlights on the capacities of “Gel-based” proteomics. Proteome Sci 2010; 8: 23. Liumbruno G, D’Alessandro A, Grazzini G, Zolla L. Blood- related proteomics. J Proteomics 2010; 73: 483–507.
  10. Herosimczyk A, Lepczyński A, Dratwa-Chałupnik A, Kurpińska A, Klonowska A, Skrzypczak WF. Age-related changes of se