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Dergiler / Journal of Basic and Clinical Health Sciences / 2026 / Volume: 9 Issue: 1

Investigation of Epidermal Growth Factor Receptor Pathway Related, Exosomal miRNAs in Non-Small Cell Lung Cancer Cases

Altuğ Koç(İzmir Şehir Hastanesi)0000-0002-8366-6806
Asım Leblebici(DOKUZ EYLÜL ÜNİVERSİTESİ)0000-0002-5197-6631
Haydar Soydaner Karakuş(EGE ÜNİVERSİTESİ, EGE TIP FAKÜLTESİ)0000-0002-3099-9172
Ceyda Aldağ(EGE ÜNİVERSİTESİ, EGE TIP FAKÜLTESİ)0000-0003-3130-4739
Kadri Murat Erdoğan(İzmir Şehir Hastanesi)0000-0003-0097-1424
Merve Saka Güvenç(İzmir Şehir Hastanesi)0000-0001-8842-0381
Su Özgür(EGE ÜNİVERSİTESİ, EGE TIP FAKÜLTESİ)0000-0002-8993-674X
Tuncay Göksel(EGE ÜNİVERSİTESİ, EGE TIP FAKÜLTESİ)0000-0002-6089-1840
Yasemin Başbınar(DOKUZ EYLUL UNIVERSITY)0000-0001-9439-2217
Sayfa
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Yayın Tarihi
2025-01-31
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

In our study, the exosomal miR-30c, and miR-22-3p are successfully derived from the plasma of cancer cases and they could be potential biomarkers.

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Amaç & Hipotez:Background and Purpose: Recently, the “Epidermal Growth Factor Receptor” gene and its related pathways are potential targets in precision medicine for “Non-Small Cell Lung Cancer” cases.
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Temel Bulgular & Veri:We aim to investigate, 4 exosomal miRNAs: miR-30b, miR-30c, miR-221-3p, miR-22-3p; and miR-1288 as control.
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Sonuç & Katkı:In our study, the exosomal miR-30c, and miR-22-3p are successfully derived from the plasma of cancer cases and they could be potential biomarkers.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:EGFR · exosome · liquid biopsy

Abstract

Background and Purpose: Recently, the “Epidermal Growth Factor Receptor” gene and its related pathways are potential targets in precision medicine for “Non-Small Cell Lung Cancer” cases. The presented study is a proof of concept study, which utilizes exosomal miRNAs as an easy and cheap liquid biopsy approach. We aim to investigate, 4 exosomal miRNAs: miR-30b, miR-30c, miR-221-3p, miR-22-3p; and miR-1288 as control. They are defined in the literature previously. Methods: Thirty-six samples from distinct Non-Small Cell Lung Cancer cases are analyzed. Exosomes are extracted from the plasma of the patients; miRNA isolation, cDNA synthesis, and real-time quantitative polymerase chain reactions are done and ∆/∆ Ct method is used for relative quantification. Results: The control miR1288 is expressed in all of the samples except one. The two miRNA targets miR-30c and miR-22-3p produced polymerase chain reaction curves successfully. The remaining two miRNAs (miR-30b, miR-221-3p) produce weak polymerase chain reaction curves. Conclusion: miR-30b, miR-221-3p, miR-30c, and miR-22-3p are reported to be associated with lung cancer previously, but they have not been studied in a patient series before. In our study, the exosomal miR-30c, and miR-22-3p are successfully derived from the plasma of cancer cases and they could be potential biomarkers.

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Bu Makaleye Atıf Yap

Koç, A., Leblebici, A., Karakuş, H. S., Aldağ, C., Erdoğan, K. M., Güvenç, M. S., Özgür, S., Göksel, T., Başbınar, Y. (2025). Investigation of Epidermal Growth Factor Receptor Pathway Related, Exosomal miRNAs in Non-Small Cell Lung Cancer Cases. Journal of Basic and Clinical Health Sciences, Volume: 9 Issue: 1. https://doi.org/10.30621/jbachs.1595106

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