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Dergiler / ENT Updates / 2026 / Cilt: 9 Sayı: 2

Metformin And Dichloroacetate Combination Exert A Synergistic Effect On Cell Viability Of Oral Squamous Cell Carcinoma

Seniz Inanc(Dokuz Eylul University, Institute of Health Sciences. Department of Medical Biochemistry)0000-0002-4985-9022
Didem Keles(Izmir University of Economics, Vocational School of Health Services, Department of Medical Laboratory Techniques)0000-0001-7350-8049
Gorkem Eskiizmir(Manisa Celal Bayar University, Faculty of Medicine, Department of Otolaryngology-Head and Neck Surgery)0000-0002-3125-8288
Yasemin Basbinar(Dokuz Eylul University, Institute of Oncology, Department of Translational Oncology)0000-0001-9439-2217
Gulgun Oktay(Dokuz Eylul University, School of Medicine, Department of Medical Biochemistry)0000-0002-0481-6029
Sayfa
68–73
Yayın Tarihi
2019-08-08
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Therefore, a combined application of Metformin and DCA may be considered as a candidate therapy for the “drug repositioning” of the treatment of oral cavity cancer.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: To assess the effects of Metformin, Dichloroacetate (DCA) and their combination on cell viability in oral squamous cell carcinoma, UPCI-SCC-131 cell line.
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Temel Bulgular & Veri:Methods: UPCI-SCC-131 cells were plated in 96 E-plate (1x104 cells/well) and were treated with Metformin (1-16mM) and/or DCA (15-120mM) for 24-48-72h. xCELLigence SP system was used to monitor real time cell viability.
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Sonuç & Katkı:Therefore, a combined application of Metformin and DCA may be considered as a candidate therapy for the “drug repositioning” of the treatment of oral cavity cancer.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Metformin · Dichloroacetate · Drug combination

Abstract

Objective: To assess the effects of Metformin, Dichloroacetate (DCA) and their combination on cell viability in oral squamous cell carcinoma, UPCI-SCC-131 cell line. Methods: UPCI-SCC-131 cells were plated in 96 E-plate (1x104 cells/well) and were treated with Metformin (1-16mM) and/or DCA (15-120mM) for 24-48-72h. xCELLigence SP system was used to monitor real time cell viability. In addition, drug combination index was analyzed with CompuSyn software according to Chou-Talalay method. Results: Half-maximal inhibitory concentrations (IC50) of Metformin and DCA were found to be 3mM and 23mM, respectively, for 72 hours. CI values (0.76-0.80) in all combination groups below 1 indicated that Metformin/DCA combination had a moderate synergistic effect on cell viability in UPCI-SCC-131 cells. Discussion: Metformin/DCA combination synergistically decreased the cell viability of UPCI-SCC-131 cells. Therefore, a combined application of Metformin and DCA may be considered as a candidate therapy for the “drug repositioning” of the treatment of oral cavity cancer.

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Bu Makaleye Atıf Yap

Inanc, S., Keles, D., Eskiizmir, G., Basbinar, Y., Oktay, G. (2019). Metformin And Dichloroacetate Combination Exert A Synergistic Effect On Cell Viability Of Oral Squamous Cell Carcinoma. ENT Updates, Cilt: 9 Sayı: 2, 68–73. https://doi.org/10.32448/entupdates.569464

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