Dergiler / Turkish Journal of Medical Sciences / 2016 / Cilt: 46 - Sayı: 3

Investigation of enteropathogenic Escherichia coli and Shiga toxin-producing Escherichia coli associated with hemolytic uremic syndrome in İzmir Province, Turkey

Sayfa
733–741
Yayın Tarihi
2016-06-01
DOI
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Conclusion: To the best of our knowledge, this is the first report of O174:H21 from the İzmir region that was shown to be a Shiga toxin-producing non-O157 serotype of STEC.

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Amaç & Hipotez:Background/aim: The purpose of this study was to investigate Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) strains originating from diarrheagenic patients.
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Temel Bulgular & Veri:Materials and methods: A total of 102 patients with diarrhea between October 2012 and January 2013 were enrolled in this study.
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Sonuç & Katkı:Conclusion: To the best of our knowledge, this is the first report of O174:H21 from the İzmir region that was shown to be a Shiga toxin-producing non-O157 serotype of STEC.
🔬Tür:Araştırma Makalesi
📍Kapsam:2012 Verileri
⚙️Değişkenler:Enteropathogenic Escherichia coli · Shiga toxin-producing Escherichia coli · diarrhea

Abstract

Background/aim: The purpose of this study was to investigate Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) strains originating from diarrheagenic patients. Materials and methods: A total of 102 patients with diarrhea between October 2012 and January 2013 were enrolled in this study. Multiplex and standard polymerase chain reactions were performed to detect and distinguish STEC and EPEC strains. O serotyping of EPEC was carried out by monovalent antisera. The O and H serotyping of STEC strains was performed at the Refik Saydam Institute, Ankara. Results: A total of 5 (3.42%) strains were identified as STEC, and 3 strains (2.05%) were atypical EPEC. One of the STEC serotypes was O157:H7 carrying VT1, Stx1A, and escv genes. The other STEC strain was identified as O174:H21, which is associated with hemolytic uremic syndrome and consists of VT2 and Stx2A genes. One of the EPEC and three of the STEC serotypes were nontypeable. The serotypes of the atypical EPEC strains were identified as O114 and O26. Conclusion: To the best of our knowledge, this is the first report of O174:H21 from the İzmir region that was shown to be a Shiga toxin-producing non-O157 serotype of STEC.

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Bu Makaleye Atıf Yap

BOZÇAL, E., YİĞİTTÜRK, G., UZEL, A., AYDEMİR, S. Ş. (2016). Investigation of enteropathogenic Escherichia coli and Shiga toxin-producing Escherichia coli associated with hemolytic uremic syndrome in İzmir Province, Turkey. Turkish Journal of Medical Sciences, Cilt: 46 - Sayı: 3, 733–741. https://ajindex.com/articles/113891

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