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Dergiler / Turkish Journal of Medical Sciences / 2018 / Cilt: 48 - Sayı: 6

Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species

İşın AKYAR
Cengiz ÇAVUŞOĞLU
Meltem AYAŞ
Süheyla SÜRÜCÜOĞLU
Arzu İLKİ
Deniz Ece KAYA
Yeşim BEŞLİ
Sayfa
1351–1357
Yayın Tarihi
2018-12-12
DOI
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Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.

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Amaç & Hipotez:Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules.
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Temel Bulgular & Veri:In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis.
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Sonuç & Katkı:Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:MALDI-TOF MS · identification · Mycobacterium

Abstract

Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis. Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculous mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study. Results: Identification by MBT ML4.0 was correctly performed in 100% of MTC and in 91% of NTM isolates. All of the MTC isolates were correctly differentiated from NTM isolates. Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.

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Bu Makaleye Atıf Yap

AKYAR, İ., ÇAVUŞOĞLU, C., AYAŞ, M., SÜRÜCÜOĞLU, S., İLKİ, A., KAYA, D. E., BEŞLİ, Y. (2018). Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species. Turkish Journal of Medical Sciences, Cilt: 48 - Sayı: 6, 1351–1357. https://ajindex.com/articles/113881

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Kaynakça (8)

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