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Dergiler / Turkish Journal of Medical Sciences / 2015 / Cilt: 45 - Sayı: 6

Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience

Cem ÇELİK
Mustafa Gökhan GÖZEL
Mustafa Zahir BAKICI
Serdar BERK
Sefa Levent ÖZŞAHİN
Esra GÜLTÜRK
Sayfa
1329–1334
Yayın Tarihi
2015-12-04
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Its usefulness in culture-negative patients and the best method for integrating this diagnostic method into current tuberculosis diagnostic algorithms both need further study.

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Amaç & Hipotez:Background/aim: The aim of this study is to assess the Xpert MTB/RIF assay for diagnosis of the Mycobacterium tuberculosis complex in clinical samples and to compare the results by reference to the diagnostic method, Bactec MGIT 960.
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Temel Bulgular & Veri:Materials and methods: A total of 7407 samples were included from patients not primarily suggesting pulmonary or extrapulmonary tuberculosis (TB), collected from patients required to be screened for TB and excluding TB diagnoses since it was a differential diagnosis.
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Sonuç & Katkı:Its usefulness in culture-negative patients and the best method for integrating this diagnostic method into current tuberculosis diagnostic algorithms both need further study.
🔬Tür:Araştırma Makalesi
📍Kapsam:7407 samples
⚙️Değişkenler:Tuberculosis diagnosis · Mycobacterium tuberculosis complex · Xpert MTB/RIF assay

Özet

Background/aim: The aim of this study is to assess the Xpert MTB/RIF assay for diagnosis of the Mycobacterium tuberculosis complex in clinical samples and to compare the results by reference to the diagnostic method, Bactec MGIT 960. Materials and methods: A total of 7407 samples were included from patients not primarily suggesting pulmonary or extrapulmonary tuberculosis (TB), collected from patients required to be screened for TB and excluding TB diagnoses since it was a differential diagnosis. Also included were a total of 411 samples from patients primarily suggesting pulmonary or extrapulmonary TB. Results: In the first group, 152 of 7407 samples yielded positive results with the Bactec MGIT 960, 131 (1.77%) were found positive with Löwenstein-Jensen medium, and 295 (3.99%) were found positive with Ziehl-Neelsen staining. In the second group, 24 (5.8%), 17 (4.1%), and 28 (6.8%) of 411 samples were found positive. Xpert MTB/RIF [27 (6.6%) of 411 samples] detected 3 additional samples as positive, and these 3 cases were clinically compatible with TB. Conclusion: The Xpert MTB/RIF assay shows superior performance for the diagnosis of TB. Its usefulness in culture-negative patients and the best method for integrating this diagnostic method into current tuberculosis diagnostic algorithms both need further study.

Abstract

Background/aim: The aim of this study is to assess the Xpert MTB/RIF assay for diagnosis of the Mycobacterium tuberculosis complex in clinical samples and to compare the results by reference to the diagnostic method, Bactec MGIT 960. Materials and methods: A total of 7407 samples were included from patients not primarily suggesting pulmonary or extrapulmonary tuberculosis (TB), collected from patients required to be screened for TB and excluding TB diagnoses since it was a differential diagnosis. Also included were a total of 411 samples from patients primarily suggesting pulmonary or extrapulmonary TB. Results: In the first group, 152 of 7407 samples yielded positive results with the Bactec MGIT 960, 131 (1.77%) were found positive with Löwenstein-Jensen medium, and 295 (3.99%) were found positive with Ziehl-Neelsen staining. In the second group, 24 (5.8%), 17 (4.1%), and 28 (6.8%) of 411 samples were found positive. Xpert MTB/RIF [27 (6.6%) of 411 samples] detected 3 additional samples as positive, and these 3 cases were clinically compatible with TB. Conclusion: The Xpert MTB/RIF assay shows superior performance for the diagnosis of TB. Its usefulness in culture-negative patients and the best method for integrating this diagnostic method into current tuberculosis diagnostic algorithms both need further study.

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Bu Makaleye Atıf Yap

ÇELİK, C., GÖZEL, M. G., BAKICI, M. Z., BERK, S., ÖZŞAHİN, S. L., GÜLTÜRK, E. (2015). Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience. Turkish Journal of Medical Sciences, Cilt: 45 - Sayı: 6, 1329–1334. https://ajindex.com/articles/113749

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Kaynakça (12)

  1. Xu HB, Jiang RB, Li L. Pulmonary resection for patients with multidrug-resistant tuberculosis: systematic review and meta- analysis. J Antimicrob Chemoth 2011; 66: 1687–1695.
  2. Nikam C, Jagannath M, Narayanan MM, Ramanabhiraman V, Kazi M, Shetty A, Rodrigues C. Rapid diagnosis of Mycobacterium tuberculosis with Truenat MTB: a near-care approach. PLoS One 2013; 8: e51121. Baylan O. Extensively drug resistant and extremely drug resistant tuberculosis forms after multi-drug resistant tuberculosis: new faces of the old disease. Mikrobiyol Bul 2011; 45: 181–195 (in Turkish with abstract in English). 4. Chang K, Lu W, Wang J, Zhang K, Jia S, Li F, Deng S, Chen M. Rapid and effective diagnosis of tuberculosis and rifampicin resistance with Xpert MTB/RIF assay: a meta-analysis. J Infect 2012; 64: 580–588.
  3. Weyer K, Carai S, Nunn P. Viewpoint TB diagnostics: what does the world really need? J Infect Dis 2011; 204: 1196–1202.
  4. Hillemann D, Ruesch-Gerdes S, Boehme C, Richter E. Rapid molecular detection of extrapulmonary tuberculosis by automated GeneXpert® MTB/RIF system. J Clin Microbiol 2011; 49: 1202–1205. WHO. Roadmap for Rolling Out Xpert MTB/RIF for Rapid Diagnosis of TB and MDR-TB. Geneva, Switzerland: World Health Organization; 2010. Dorman SE. New diagnostic tests for tuberculosis: bench, bedside, and beyond. Clin Infect Dis 2010; 50: 173–177.
  5. Takashima T, Higuchi T. Mycobacterial tests. Kekkaku 2008; 83: 43–59.
  6. Chien HP, Yu MC, Wu MH, Lin TP, Luh KT. Comparison of the BACTEC MGIT 960 with Löwenstein–Jensen medium for recovery of mycobacteria from clinical specimens. Int J Tuberc Lung Dis 2000; 4: 866–870.
  7. Ray S, Talukdar A, Kundu S, Khanra D, Sonthalia N. Diagnosis and management of miliary tuberculosis: current state and future perspectives. Ther Clin Risk Manag 2013; 9: 9–26.
  8. Naing C, Mak JW, Maung M, Wong SF, Kassim AI. Meta- analysis: the association between HIV infection and extrapulmonary tuberculosis. Lung 2013; 191: 27–34.
  9. Nakazawa Y, Nishino T, Mori A, Uramatsu T, Obata Y, Arai H, Hayashi H, Tsukasaki S, Muraya Y, Inoue Y et al. Tuberculous osteomyelitis in the ulna of a patient undergoing hemodialysis. Intern Med 2013; 52: 135–139.
  10. Hristea A, Olaru ID, Baicus C, Moroti R, Arama V, Ion M. Clinical prediction rule for differentiating tuberculous from viral meningitis. Int J Tuberc Lung Dis 2012; 16: 793–798.
  11. Liu A, Nicol E, Hu Y, Coates A. Tuberculous endocarditis. Int J Cardiol 2013; 167: 640–645.
  12. Kucukardali Y, Oncul O, Cavuslu S, Danaci M, Calangu S