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İNCİR ÇEKİRDEĞİ YAĞININ KOLOREKTAL KANSER HÜCRE HATLARI ÜZERİNDEKİ SİTOTOKSİK VE ANTİ-İNVAZİV ETKİLERİ

EFFECTS OF FIG SEED OIL ON THE PROLIFERATION AND INVASION OF COLORECTAL CANCER CELL LINES

Zeynep Doğru(SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE DİŞ HEKİMLİĞİ FAKÜLTESİ, TEMEL BİLİMLER BÖLÜMÜ, TEMEL TIP BİLİMLERİ ANABİLİM DALI)0000-0001-7115-3360
Mehmet Akbulut(SELÇUK ÜNİVERSİTESİ)0000-0001-5621-8293
Hüsamettin Vatansev(SELÇUK ÜNİVERSİTESİ)0000-0002-0230-3414
Serdar Karakurt(SELÇUK ÜNİVERSİTESİ)0000-0002-4449-6103
Sayfa
878–887
Yayın Tarihi
2025-09-19
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bulgular, incir çekirdeği yağının antikanser potansiyel taşıyan doğal bir ajan olabileceğini göstermektedir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Amaç: Kolorektal kanser (KRC), dünya genelinde yüksek morbidite ve mortaliteye sahip yaygın bir malignitedir.
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Temel Bulgular & Veri:Bu çalışma, incir (Ficus carica) çekirdeği yağının HT-29 ve DLD-1 kolorektal kanser hücre hatlarında proliferasyon ve invazyon üzerindeki etkilerinin in vitro koşullarda değerlendirilmesini amaçlamaktadır.
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Sonuç & Katkı:Bulgular, incir çekirdeği yağının antikanser potansiyel taşıyan doğal bir ajan olabileceğini göstermektedir.
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:İncir çekirdeği yağı · invazyon · kolorektal kanser

Özet

Amaç: Kolorektal kanser (KRC), dünya genelinde yüksek morbidite ve mortaliteye sahip yaygın bir malignitedir. Cerrahi ve kemoterapi gibi geleneksel tedavilerin yanında, doğal kaynaklı biyoaktif bileşiklerin destekleyici terapötik potansiyelleri üzerine çalışmalar giderek artmaktadır. Bu çalışma, incir (Ficus carica) çekirdeği yağının HT-29 ve DLD-1 kolorektal kanser hücre hatlarında proliferasyon ve invazyon üzerindeki etkilerinin in vitro koşullarda değerlendirilmesini amaçlamaktadır. Gereç ve Yöntem: İncir çekirdeği yağının kimyasal bileşimi GC-FID ve HPLC analizleri ile karakterize edilmiştir. Sitotoksik etkiler Alamar Blue testi ile değerlendirilmiş ve IC₅₀ değerleri hesaplanmıştır; invazyon kapasitesi ise Matrigel transwell invazyon analizi ile belirlenmiştir. Sonuç ve Tartışma: Analiz sonuçlarına göre, incir çekirdeği yağı yüksek düzeyde α-linolenik asit (%39.97), linoleik asit (%33.24), oleik asit (%16.71) ve γ-tokoferol (4800 mg/kg) içermektedir. Yağ, HT-29 ve DLD-1 hücrelerinde doza bağlı antiproliferatif etki göstermiş, sağlıklı PNT1A hücrelerinde ise daha düşük toksisite sergilemiştir. Ayrıca, her iki kanser hücre hattında anlamlı düzeyde invazyon inhibisyonu saptanmıştır. Bulgular, incir çekirdeği yağının antikanser potansiyel taşıyan doğal bir ajan olabileceğini göstermektedir.

Abstract

Objective: Colorectal cancer (CRC) is a common malignancy associated with high morbidity and mortality worldwide. In addition to conventional treatments such as surgery and chemotherapy, there is a growing interest in the therapeutic potential of naturally derived bioactive compounds. This study aims to evaluate the in vitro effects of fig (Ficus carica) seed oil on the proliferation and invasion of HT-29 and DLD-1 colorectal cancer cell lines. Material and Method: The chemical composition of fig seed oil was characterized by GC-FID and HPLC analyses. Cytotoxic effects were assessed using the Alamar Blue assay, and IC₅₀ values were calculated. Invasion capacity was determined through a Matrigel-transwell invasion assay. Result and Discussion: According to the analysis results, fig seed oil was found to be rich in α-linolenic acid (39.97%), linoleic acid (33.24%), oleic acid (16.71%), and γ-tocopherol (4800 mg/kg). The oil exhibited dose-dependent antiproliferative activity on HT-29 and DLD-1 cells, while demonstrating lower toxicity on healthy PNT1A cells. Furthermore, a significant inhibition of invasion was observed in both cancer cell lines. These findings suggest that fig seed oil may serve as a natural agent with anticancer potential for use in CRC management.

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Bu Makaleye Atıf Yap

Doğru, Z., Akbulut, M., Vatansev, H., Karakurt, S. (2025). İNCİR ÇEKİRDEĞİ YAĞININ KOLOREKTAL KANSER HÜCRE HATLARI ÜZERİNDEKİ SİTOTOKSİK VE ANTİ-İNVAZİV ETKİLERİ. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 49 Sayı: 3, 878–887. https://doi.org/10.33483/jfpau.1650925

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