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Dergiler / Ankara Üniversitesi Eczacılık Fakültesi Dergisi / 2026 / Cilt: 49 Sayı: 3

ORİGANUM ONİTES L. ETANOLİK EKSTRAKTI OKSİDAN, İNFLAMATUAR VE APOPTOTİK BELİRTEÇLERİ DÜZENLEYEREK SIÇANLARDA ASETİK ASİT İLE İNDÜKLENEN ÜLSERATİF KOLİTİ HAFİFLETİR

ORIGANUM ONITES L. ETHANOLIC EXTRACT ALLEVIATES ACETIC ACID-INDUCED ULCERATIVE COLITIS IN RATS BY REGULATING OXIDANT, INFLAMMATORY, AND APOPTOTIC MARKERS

Dilek Özbeyli(MARMARA ÜNİVERSİTESİ, SAĞLIK HİZMETLERİ YÜKSEKOKULU)0000-0002-4141-6913
Ali Şen(MARMARA UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF PROFESSIONAL PHARMACEUTICAL SCIENCES, DEPARTMENT OF PHARMACOGNOSY)0000-0002-2144-5741
Leyla Bitiş(MARMARA UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF PROFESSIONAL PHARMACEUTICAL SCIENCES, DEPARTMENT OF PHARMACOGNOSY)0000-0003-1167-6666
Naziye Özkan Yenal(MARMARA ÜNİVERSİTESİ, SAĞLIK HİZMETLERİ MESLEK YÜKSEKOKULU)0000-0003-3524-0672
Ayşe Nur Hazar Yavuz(MARMARA ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ ENSTİTÜSÜ, FARMAKOLOJI (DR) (ECZACILIK))0000-0003-0784-8779
Deniz Mukaddes Türet(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE)0000-0003-1989-9637
Meral Yüksel(MARMARA ÜNİVERSİTESİ, SAĞLIK HİZMETLERİ MESLEK YÜKSEKOKULU)0000-0002-4760-3306
Ahmet Doğan(MARMARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, ECZACILIK TEKNOLOJİSİ BÖLÜMÜ, FARMASÖTİK BOTANİK ANABİLİM DALI)0000-0003-0603-5100
Aslı Aykaç(NEAR EAST UNIVERSITY)0000-0002-4885-5070
Sayfa
631–650
Yayın Tarihi
2025-09-19
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Sonuç olarak, O. onites EE'nin sıçanlarda kolit üzerinde iyi bir terapötik etkisi vardır; bu da esas olarak total flavonoid, fenolik ve triterpen içeriği ve Anti-5-LOX ve serbest radikal temizleyici aktivitesinden kaynaklanmaktadır.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Amaç: Bu çalışmada Origanum onites etanolik özütünün (O. onites EE) antioksidan ve anti-inflamatuar etkileri in vitro olarak ve inflamasyon, apoptoz, oksidatif stres ve doku hasarıyla ilişkili parametreler üzerindeki etkileri asetik asit (AA) ile indüklenen sıçan ülseratif kolit (UC) modelinde değerlendirilmiştir.
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Temel Bulgular & Veri:Daha sonra, ekstrenin anti-DPPH ve anti-ABTS serbest radikal temizleme, anti-5-lipoksijenaz aktiviteleri ve toplam sekonder metabolik miktarları ölçüldü.
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Sonuç & Katkı:Sonuç olarak, O. onites EE'nin sıçanlarda kolit üzerinde iyi bir terapötik etkisi vardır; bu da esas olarak total flavonoid, fenolik ve triterpen içeriği ve Anti-5-LOX ve serbest radikal temizleyici aktivitesinden kaynaklanmaktadır.
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Anti-5-LOX · apoptoz · IL-17

Özet

Amaç: Bu çalışmada Origanum onites etanolik özütünün (O. onites EE) antioksidan ve anti-inflamatuar etkileri in vitro olarak ve inflamasyon, apoptoz, oksidatif stres ve doku hasarıyla ilişkili parametreler üzerindeki etkileri asetik asit (AA) ile indüklenen sıçan ülseratif kolit (UC) modelinde değerlendirilmiştir. Gereç ve Yöntem: O. onites'in kurutulmuş kısımları maserasyon yöntemi kullanılarak etanol ile ekstre edildi. Daha sonra, ekstrenin anti-DPPH ve anti-ABTS serbest radikal temizleme, anti-5-lipoksijenaz aktiviteleri ve toplam sekonder metabolik miktarları ölçüldü. Sıçan grupları kontrol + salin (C), UC + salin (A), UC + O. onites EE (O), UC + Sülfasalazin (S) olarak belirlendi. Tüm tedaviler 3 ardışık gün boyunca günde bir kez uygulandı. Sonuç ve Tartışma: O. onites EE, toplam fenolik, flavonoid ve triterpen içeriklerine iyi miktarda, sahipti ve ABTS, DPPH radikallerine karşı yüksek bir antioksidan aktivite ve 5-LOX enzimine karşı yüksek bir anti-inflamatuar aktivite gösterdi. AA ile uygulanması ile artan luminol-lusigenin, TNF-α, IL-1, IL-17, TLR-9, kaspaz-3, kaspaz-9, MMP-3, makroskobik ve mikroskobik skorlar, O. onites EE ve sülfasalazin tedavileriyle azaldı. Sonuç olarak, O. onites EE'nin sıçanlarda kolit üzerinde iyi bir terapötik etkisi vardır; bu da esas olarak total flavonoid, fenolik ve triterpen içeriği ve Anti-5-LOX ve serbest radikal temizleyici aktivitesinden kaynaklanmaktadır.

Abstract

Objective: This study aims to evaluate the antioxidant and anti-inflammatory effects of Origanum onites ethanolic extract (O. onites EE) in vitro and its effects on inflammation, apoptosis, oxidative stress, and tissue damage-related parameters in the acetic acid (AA)-induced ulcerative colitis (UC) model in rats. Material and Method: Dried parts of O. onites were extracted with ethanol using the maceration method. Then, the extract’s anti-DPPH and anti-ABTS free radical scavenging, anti-5-lipoxygenase (LOX) activities, and total secondary metabolic quantities were evaluated in vitro. The rat groups were determined as control + saline (C), AA + saline (A), AA + O. onites EE (O), AA + Sulfasalazine (S). The treatments were applied once a day for 3 consecutive days. Result and Discussion: O. onites EE showed high amount of total phenolic, total flavonoid, and total triterpene contents, high antioxidant activity against ABTS, DPPH radicals, and anti-inflammatory effects against 5-LOX enzyme. Due to AA-induction, increased luminol-lucigenin, TNF-α, IL-1β, IL-17, TLR-9, caspase-3, caspase-9, MMP-3, macroscopic and microscopic scores attenuated by O. onites EE and sulfasalazine treatments. In conclusion, O. onites EE has a significant therapeutic effect on rats' colitis due to its total flavonoid, phenolic, and triterpene content, and its Anti-5-LOX and free radical scavenging activity.

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Bu Makaleye Atıf Yap

Özbeyli, D., Şen, A., Bitiş, L., Yenal, N. Ö., Yavuz, A. N. H., Türet, D. M., Yüksel, M., Doğan, A., Aykaç, A. (2025). ORİGANUM ONİTES L. ETANOLİK EKSTRAKTI OKSİDAN, İNFLAMATUAR VE APOPTOTİK BELİRTEÇLERİ DÜZENLEYEREK SIÇANLARDA ASETİK ASİT İLE İNDÜKLENEN ÜLSERATİF KOLİTİ HAFİFLETİR. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 49 Sayı: 3, 631–650. https://doi.org/10.33483/jfpau.1582539

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