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Dergiler / Bitki Koruma Bülteni / 2025 / Cilt: 65 Sayı: 4

Buğdaydan elde edilen Rhizoctonia anastomosis gruplarının moleküler karakterizasyonu

Molecular characterization of Rhizoctonia anastomosis groups from wheat

Harun Bayraktar(ANKARA ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ)0000-0003-2562-4461
Filiz Ünal(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ)0000-0003-4620-5397
Fatma Sara Dolar(ANKARA ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ)0000-0003-1634-4046
Sayfa
100–110
Yayın Tarihi
2026-01-01
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Farklı grup ve alt gruptaki izolatlar ise farklı dallarda yer alarak grup ve altgruplar arasındaki genetik farklılığı ortaya koymuştur.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Orta Anadolu buğday tarlalarından toplanan bitki ve toprak örneklerinden izole edilen Rhizoctonia izolatları MseI, Ava II/HincII, MunI, MspI (HapII), HinfI, EcoRI, HaeIII, HinfI ve HhaI enzimleri kullanılarak PCR-RFLP ve DNA sekans analizine tabi tutulmuştur.
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Temel Bulgular & Veri:Analiz sonucunda Rhizoctonia izolatlarının çok çekirdekli Rhizoctonia solani AG 2-1, AG 2 BI, AG 3, AG 4 HG II, AG 5, AG 8 ve Waitea circinata var. circinata, Waitea circinata var. zeae, Waitea circinata var. oryzae grup I, çift çekirdekli Rhizoctonia AG DI, AG E, AG G, AG I ve AG K olduğu belirlenmiştir.
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Sonuç & Katkı:Farklı grup ve alt gruptaki izolatlar ise farklı dallarda yer alarak grup ve altgruplar arasındaki genetik farklılığı ortaya koymuştur.
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Rhizoctonia · anastomosis grup · PCR

Özet

Orta Anadolu buğday tarlalarından toplanan bitki ve toprak örneklerinden izole edilen Rhizoctonia izolatları MseI, Ava II/HincII, MunI, MspI (HapII), HinfI, EcoRI, HaeIII, HinfI ve HhaI enzimleri kullanılarak PCR-RFLP ve DNA sekans analizine tabi tutulmuştur. Analiz sonucunda Rhizoctonia izolatlarının çok çekirdekli Rhizoctonia solani AG 2-1, AG 2 BI, AG 3, AG 4 HG II, AG 5, AG 8 ve Waitea circinata var. circinata, Waitea circinata var. zeae, Waitea circinata var. oryzae grup I, çift çekirdekli Rhizoctonia AG DI, AG E, AG G, AG I ve AG K olduğu belirlenmiştir. Filogenetik ağaçlarda aynı grup ve altgrupta yer alan izolatlar aynı grupta yer almışlardır. Farklı grup ve alt gruptaki izolatlar ise farklı dallarda yer alarak grup ve altgruplar arasındaki genetik farklılığı ortaya koymuştur.

Abstract

Rhizoctonia isolates isolated from plant and soil samples collected from Central Anatolian wheat fields were subjected to PCR-RFLP and DNA sequence analysis using MseI, Ava II/HincII, MunI, MspI (HapII), HinfI, EcoRI, HaeIII, HinfI and HhaI enzymes. As a result of the analysis, Rhizoctonia isolates were determined to be multinucleate Rhizoctonia solani AG 2-1, AG 2 BI, AG 3, AG 4 HG II, AG 5, AG 8, and Waitea circinata var. circinata, Waitea circinata var. zeae, Waitea circinata var. oryzae group I, binucleate Rhizoctonia AG DI, AG E, AG G, AG I and AG K. Isolates in the same group and subgroup in phylogenetic trees were in the same group. Isolates from different groups and subgroups were located in various branches, revealing the genetic differences between the groups and subgroups.

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Bu Makaleye Atıf Yap

Bayraktar, H., Ünal, F., Dolar, F. S. (2026). Buğdaydan elde edilen Rhizoctonia anastomosis gruplarının moleküler karakterizasyonu. Bitki Koruma Bülteni, Cilt: 65 Sayı: 4, 100–110. https://doi.org/10.16955/bitkorb.1551947

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