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Dergiler / European Journal of Biology / 2023 / Volume: 82 Issue: 2

Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells

Burak Durmaz(Ege University Faculty of Medicine, Department of Medical Biochemistry)0000-0002-6383-6724
Latife Merve Oktay(Ege University, Faculty of Medicine, Department of Medical Biology)0000-0002-8012-0445
Hikmet Memmedov(Azerbaijan University of Medicine; Department of Medical Biochemistry)0000-0002-8012-0445
Nur Selvi Günel(Ege University, Faculty of Medicine, Department of Medical Biology)0000-0002-6383-6724
Hatice Kalkan Yıldırım(Ege University, Faculty of Engineering, Department of Food Engineering)0000-0001-9698-9682
Eser Y. Sözmen(Ege University Faculty of Medicine, Department of Medical Biochemistry)0000-0002-6383-6724
Sayfa
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Yayın Tarihi
2023-12-21
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Conclusion: In samples that were dissolved in water, dissolved in ethanol+biotransformed with L. plantarum ATCC®8014, dissolved in water+biotransformed with L. plantarum ATCC®8014, and dissolved in water+sonicated for 15 min and biotransformed with L. plantarum ATCC®8014, the maximum anti-inflammatory effect of propolis was assessed.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: Propolis has been found to have various effects, including antioxidant and anti-inflammatory properties, according to studies.
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Temel Bulgular & Veri:The study aimed to identify the extraction methods and solvents that had the most significant anti-inflammatory effects and assess how L. plantarum strains biotransformation of propolis affected these qualities in THP-1 cell line cultures.
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Sonuç & Katkı:Conclusion: In samples that were dissolved in water, dissolved in ethanol+biotransformed with L. plantarum ATCC®8014, dissolved in water+biotransformed with L. plantarum ATCC®8014, and dissolved in water+sonicated for 15 min and biotransformed with L. plantarum ATCC®8014, the maximum anti-inflammatory effect of propolis was assessed.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:LPS · inflammation · propolis

Abstract

Objective: Propolis has been found to have various effects, including antioxidant and anti-inflammatory properties, according to studies. In this recent research, we discovered that reducing allergenic compounds in propolis through biotransformation using specific Lactobacillus plantarum strains enhanced its anti-inflammatory qualities. The study aimed to identify the extraction methods and solvents that had the most significant anti-inflammatory effects and assess how L. plantarum strains biotransformation of propolis affected these qualities in THP-1 cell line cultures. Materials and Methods: Propolis samples were biotransformed with different concentrations (1.5%, 2.5%, 3.5%) of several L. plantarum strains (ISLG-2, ATCC®8014, visbyvac) before extraction using various solvents (ethanol, polyethylene glycol-PEG, water) and ultrasound treatments (300 W/40 Hz for 5, 10, 15 min). Liquid chromatography-mass spectrometer/mass spectrometry was used for phenolic analysis of the samples. ELISA test kits were employed to assess NF-kβ, IL-1α, IL-1β, IL-6, IL-10, TNF-α, IFN-γ , COX-1 in the cell culture supernatant. Results: Results showed that, except for NF-kβ, all cytokine levels decreased in four separate propolis samples. Caffeic acid, kaempferol, ferulic acid, quercetin, pelargonin, and naringenin were the key physiologically active components associated with the anti-inflammatory activity of propolis. The biotransformation process to reduce allergen compounds did not alter propolis’s anti-inflammatory properties. Conclusion: In samples that were dissolved in water, dissolved in ethanol+biotransformed with L. plantarum ATCC®8014, dissolved in water+biotransformed with L. plantarum ATCC®8014, and dissolved in water+sonicated for 15 min and biotransformed with L. plantarum ATCC®8014, the maximum anti-inflammatory effect of propolis was assessed.

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Bu Makaleye Atıf Yap

Durmaz, B., Oktay, L. M., Memmedov, H., Günel, N. S., Yıldırım, H. K., Sözmen, E. Y. (2023). Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells. European Journal of Biology, Volume: 82 Issue: 2. https://doi.org/10.26650/EurJBiol.2023.1247199

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