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Dergiler / European Journal of Biology / 2026 / Volume: 83 Issue: 1

Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells

Gamze Nur Öter(Istanbul University, Institute of Health Sciences, Department of Medical Biochemistry)0000-0002-7063-3984
Ezgi Durmuş(Bezmialem Vakıf University, Institute of Health Sciences, Department of Medical Biochemistry)0000-0002-0760-497X
Ali Şen(MARMARA UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF PROFESSIONAL PHARMACEUTICAL SCIENCES, DEPARTMENT OF PHARMACOGNOSY)0000-0002-2144-5741
Abdurrahim Koçyiğit(BEZMI ALEM FOUNDATION UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF MEDICAL BIOCHEMISTRY)
Sayfa
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Yayın Tarihi
2024-05-30
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The results suggest R. tereticaulis leaves to have an anti-cancer effect on lung cancer cells through ROS-mediated apoptosis and RTC to be an effective therapeutic/adjuvant strategy in cancer treatment.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: Plants have been used for medicinal purposes since the beginning of human history and form the basis of modern medicine, and they are also the source of most chemotherapeutic drugs for cancer treatment.
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Temel Bulgular & Veri:This study aims to investigate for the first time the cytotoxic and apoptotic effects of the active ethanol (RTE) and chloroform (RTC) extracts of Rubus tereticaulis leaves in the A549 non-small-cell lung cancer cell line.
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Sonuç & Katkı:The results suggest R. tereticaulis leaves to have an anti-cancer effect on lung cancer cells through ROS-mediated apoptosis and RTC to be an effective therapeutic/adjuvant strategy in cancer treatment.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Lung cancer · anti-cancer effect · oxidative stress

Abstract

Objective: Plants have been used for medicinal purposes since the beginning of human history and form the basis of modern medicine, and they are also the source of most chemotherapeutic drugs for cancer treatment. This study aims to investigate for the first time the cytotoxic and apoptotic effects of the active ethanol (RTE) and chloroform (RTC) extracts of Rubus tereticaulis leaves in the A549 non-small-cell lung cancer cell line. Materials and Methods: A549 cells were treated with RTE and RTC individually. The MTT assay was used to quantitatively detect RTE and RTC’s cytotoxic effects. The fluorescent signal indicator H2DCF-DA was used to detect cellular reactive oxygen species (ROS) production. Apoptosis was evaluated by fluorescence microscope after acridine orange/ethidium bromide fluorescent staining, annexin V-FITC and immunoblotting analyses, immunofluorescence, and imaging. Results: Both RTE and RTC induced cytotoxicity in A549 cells in a dose-dependently, which was accompanied with induced ROS accumulation. Both early and late apoptotic cells detected by flow cytometry were increased in the RTE- and RTC-treated cells. In addition, the results show RTC to have higher cytotoxic and apoptotic effects and increased ROS-generation capacity than RTE. Therefore, the polarity of the solvent used to exert the anticancer effect of R. tereticaulis leaves is crucial. Conclusion: This is the first anti-cancer activity study on R. tereticaulis. The results suggest R. tereticaulis leaves to have an anti-cancer effect on lung cancer cells through ROS-mediated apoptosis and RTC to be an effective therapeutic/adjuvant strategy in cancer treatment.

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Bu Makaleye Atıf Yap

Öter, G. N., Durmuş, E., Şen, A., Koçyiğit, A. (2024). Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells. European Journal of Biology, Volume: 83 Issue: 1. https://doi.org/10.26650/EurJBiol.2024.1353842

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