Determination of Reference Intervals For Dihydrorhodamine 123 (DHR) Assay in Healthy Children

Abstract

This study aimed to determine the reference intervals for dihydrorhodamine 123 (DHR) assay in healthy children. A total of 100 healthy children, aged between 0 and 18 years and 10 adults, who admitted to Selcuk University Medical Faculty, Department of Pediatrics were included in this study. The DHR assay were evaluated in a total of 11 groups, each group consisting of 10 individuals (0-1 months, 1-3 months, 4-6 months, 7-12 months, 13-24 months, 25-36 months, 3-5 years, 6-8 years, 9-11 years, 12-18 years and adults). DHR assay was performed in peripheral blood samples with EDTA and the cells were immediately evaluated using flow cytometry. The 95% confidence interval was determined according to the mean, minimum, and maximum values obtained from this data. The stimulation index was observed to vary ranging between 21 and 451 (mean ± SD, 105.9 ± 77). When the difference between the groups was evaluated, it was found that the stimulation index was found to be low in the age group of 1-3 months to compared with the other age groups (p <0.05). The DHR assay is a reliable method to detect the levels of reactive oxygen radicals, especially hydrogen peroxide. The reference intervals of DHR assay in healthy children were determined in this study.