ISOLATION OF HIGHLY ACTIVE ANTIRADICAL AND ANTIOXIDANT POLYPHENOLICS FROM PLANT EXTRACTS USING NOVEL POLYMERIC ADSORBENT PARTICLES

Abstract

Boronic acids monomers shows optimum adsorption of diols at pH 8.5. In this study, we were able to reduce this pH to 7 by using 4- N-allylsulfamoil phenylboronic acid ASPBA monomer copolymer. Methacrylate MA and our synthesized ASPBA were used to prepare millimeter sized poly[methacrylate-co-4- N-allylsulfamoil phenylboronic acid] poly[MA-co-ASPBA] particles. These particles were characterized by 1HNMR, FTIR and TGA. The prepared particles were mixed with the extract of Hypericum perforatum L. centaury and showed highly selective interaction with the flavonoids. Antioxidant and antiradical compounds were adsorbed over the surface of the particles and later desorbed. These macroporous particles can be used in selective isolation of natural products from plant extracts, especially cis-diols. Our prepared particles can be used as packing materials in the columns to selectively capture the target polyphenolics. Alternatively, the particles can be mixed with the extracts to adsorb the target polyphenolics, and then desorb to obtain selective isolation