Validation Methods for Phenolic Components with RP-HPLC-UV in Various Bee Products

Abstract

In this study, a simple and a fast method were developed using RP-HPLC-UV for the separation and quantitative determination of phenolic compounds in various bee products. Fourteen phenolic compounds were identified using 70% acetonitrile in water, and 2% acetic acid in water as a mobile phase with a gradient elution mode. The validation method exhibited linearity (R2>0.994), limits of detection (0.022-0.062 mg/L) and quantification (0.030-0.187mg/L). Under the optimized conditions, 14 commercially available phenolic compounds were analyzed in less than 50 min. This method was successfully employed to study the phenolic profiles of bee products as well as other natural samples.