Identification of bioactive peptides in kashar cheese and its antioxidant activities

Abstract

This study analyzed the peptide profile and antioxidant activity in commercially sourced Kashar cheese. The antioxidant activity of Kashar cheese was found to be 41.09 mM Trolox g−1. However, the antioxidant activity of its F3, F4, F5 and F14 fractions was found to be 920.726 mM Trolox g−1, 545.544 mM Trolox g−1 , 783.864 mM Trolox g−1, and 392.12 mM Trolox g−1, respectively. In Kashar cheese, the Tandem Mass Spectrometry (MS/MS) spectrum for the 875 g mol−1 m/z signal was matched to αs1-casein, and it showed that 1012 g mol−1 (875+137) histidine can be a part of 1140 (1012+128) glutamine amino acid. Peptide sequences were matched to 875:RPKHPIK-H-Q peptide 1012:RPKHPIK+H peptide and 1140:RPKHPIKH+Q peptide. It can be concluded that the peptide fractions of Kashar cheese demonstrated antioxidant activity.