Miseq sequence identification of bacteria isolated from Çıldır Lake

Abstract

In this study, it was aimed to identify and phylogenetic evaluation of bacteria isolated from Çıldır lake sediment. Four sediment sampling was done from two different regions. For bacterial isolations, samples were incubated in Bennet's agar at 28 oC for 7 days. The isolated bacteria morphological and microscopic (Scaninning Electron Microscope-SEM) images were taken. After the PCR process performed with universal primers, the isolated bacteria were identified by sequencing the PCR amplicons using the MiSeq sequence method. In the results of working; 2 Nocardia sp and 1 Amycolatopsis sp species were isolated. When looking at the spore chain morphology in the SEM image, it was observed that a knobby structure was formed, the sequence alignment of 16S rRNA longer than 1000 bp and the sequences obtained from GenBank in the phylogenetic tree were closely similar (99 %