MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN

Abstract

Objective: Activation of cholinergic muscarinic receptors leads to activation of membrane - bound phospholipase C, leading to generation of IP3 and diacylglycérol, The present study aimed at measuring muscarinic receptor-mediated phosphoinositide breakdown in three different regions of rat brain.Methods: Cortical, hippocampal and striatal slices labeled with [3H] myo-inositol were incubated with muscarinic agonist carbachol (Cch) and LiCI. Reaction was terminated by the addition of chloroform/methanol and labeled inositol phosphates were extracted from tissue. Individual [3H]- inositol phosphates were separated using anion exchange chromatography.Results: Cch caused a rise in IP, formation, where the increase over basal in the cortex, hippocampus and striatum were 98%, 73% and 45%, respectively.Conclusion: Phosphoinositide turnover wasstimulated by carbachol in all brain structures studied, with the most pronounced effects observed in the cortex and hippocampus.Key Words: Muscarinic receptors, inositolphosphates, carbachol, rat brain