Sildenafilin Depresyon Benzeri Etkisinin Farelerde Zorunlu Yüzdürme Testi İle Değerlendirilmesi

Abstract

The Depressant-Like Effect of Sildenafil in The Forced Swimming Test in Mice Phosphodiesterease type 5 (PDE5), nitric oxide synthase (NOS) and guanilate cyclase have shown high activity in areas responsible to behaviours in brain. It is alleged that PDE5 inhibitor sildenafil which is used in erectile disfunction passes blood brain barrier and can cause adverse effects related to behaviours. The aim of this study, to investigate the effect of sildenanil in depression by forced swimming test in mice. We administered sildenafil (1, 5, 10, 15, 20, 30 mg/kg), sertraline (20 mg/kg) and imipramine (30 mg/kg) with intraperitoneal (ip) injection. All drugs were injected 15 min. before testing. Sertraline and imipramine given alone and in combination with sildenafil. When combination with sildenafil and other drugs were employed, other drugs were administered 10 min. before sildenafil. The activity of each mice was recorded for 5 min. in an activity cage (Ugo Basile, 7430-Varese, Italy). Then immobility was measured during the last 4 min. of the 6 min. swim test. Increase immobility time in a swim test has been found to represent "depression-like" behaviour. Sildenafil at a dose of 10mg/kg increased the immobility time without affecting locomotor activity (p