Association of CYP24A1 gene, Vitamin D deficiency and heart diseases in Pakistani patients

Abstract

Background &Aims To analyze the association of CYP24A1 gene polymorphism with vitamin D deficiency and its related cardiovascular disorders in the Pakistani population. Materials and Methods The present prospective cohort study was carried out at KRL and Rawalpindi General Hospital from January 2017 to December 2018. A total of 110 subjects suffering from heart diseases like hypertension, myocardial infarction, or congestive cardiac failure (age range 12-90 years) were enrolled in this study with informed consent. Sampling was done by non-probability convenience sampling. Blood 24Hydroxyvitmin D levels were assessed in the participants by using an electrochemiluminescence system. Genetic polymorphism in the CYP24A1 gene was screened in selected patients (n=15) through PCR-RFLP, after genomic DNA extraction from the whole blood. Data were analyzed using SPSS version 24 for Mac. Fisher’s exact test and chi-square tests were applied for qualitative variables. The risk of polymorphism in CYP24A1(SNP rs6013897) genotypes TT, AT, and AA was determined by calculating the odds ratio and confidence interval. P-value