Journals / Journal of advances in vetbio science and techniques / 2017 / Cilt: 2 - Sayı: 1
DETERMINATION OF GROWTH CHARACTERISTICS OF THE FOOT AND MOUTH VIRUSES (A, O, ASIA-1) IN BHK-21 AN30 AND BHK-21 AN73 CELL CULTURES
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- 1–5
- DOI
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Abstract
In this study, it was aimed to investigate BHK-21An73 and BHK-21An30 growth rate of cell cultures and the effects on foot and mouth disease (FMD) vaccine virus strains titers. For this purpose, growth rate of suspended cell cultures of BHK-21An73 and BHK-21An30 were determined. A TUR 11, O TUR 07 and Asia-1/11 strains of foot and mouth disease (FMD) vaccine virus strains were produced separately on BHK-21An30 and BHK-21An73. BHK-21An30 cell amounts during seven incubation days of reached to peak level in monolayer form at the 4th day with 1,3x106/ml cell numbers, in suspension form at the 4th day with 4,1x105 cell numbers. BHK-21An73 reached to peak level in monolayer form at the 6th day 2,2x106/ml cell numbers while in suspension form at the 3rd day with 2,8 x105 cell numbers. After the production of A, O and Asia-1 vaccine viruses, in suspension BHK-21An30 cell cultures average of 146S values respectively; 0,51 μg/ml, 0,18 μg/ml and 0,16 μg/ml were detected while infective titers were determined as average 6,87 (Plaque Forming Units) pfu/ml, 6,22 pfu/ml and 6,49 pfu/ml. After the production of A, O and Asia-1 vaccine viruses, in suspended BHK-21An73 cell cultures average of 146S values respectively; 2,11 μg/ml, 2,59 μg/ml and 0,53 μg/ml were detected while infective titers were determined as average 6,99 pfu/ml, 7,82 pfu/ml and 6,37 pfu/ml. As a result, in the production of A, O and Asia-1 vaccine viruses in BHK-21An73, resulted in higher 146S values and higher infective titers than BHK-21An30. It is concluded that for FMD vaccine production the using of BHK-21An73 cell culture is more appropriate for both of cost of manufacture and productive time.
Özet
Bu çalışmada, BHK-21 An73 hücre kültürü ile BHK-21 An30 hücre kültürlerinin üreme hızı, miktarı ve virus titresi üzerine etkilerinin araştırılması amaçlandı. Bu amaçla BHK-21 An30 ve BHK-21 An73 süspanse hücre kültürlerinde, hücrelerin üreme kinetikleri belirlendi. Bu hücre kültürlerinde A TUR 11, O TUR 07 ve Asia-1/11 şap aşı suşlarının üretimi gerçekleştirildi. BHK-21 An30 hücre kültürlerinin 7 günlük inkubasyon periyodu esnasında monolayer formda 4. günde 1,3x106/ml hücre sayısıyla, süspanse formda ise 4. günde 4,1x105 hücre sayısı ile pik noktaya ulaşırken BHK-21 An73 kültürlerinde ise monolayer formda 6. günde 2,2x106/ml hücre sayısına ulaşılırken, süspanse formda 3. günde 2,8 x105 hücre sayısına ulaştığı belirlendi. Süspanse BHK-21 An30 hücre kültürlerinde A, O ve Asia-1 aşı virusunun üretimi sonrası 146S değerleri sırasıyla ortalama; 0,51 μg/ml, 0,18 μg/ml ve 0,16 μg/ml olarak saptanırken enfektif titreleri ortalama 6,87 pfu/ml, 6,22 pfu/ml ve 6,49 pfu/ml olarak belirlendi. Süspanse BHK-21 An73 hücre kültürlerinde A, O ve Asia-1 aşı virusunun üretimi sonrası değerleri sırasıyla ortalama; 2,11 μg/ml, 2,59 μg/ml ve 0,53 μg/ml olarak saptanırken enfektif titreleri ortalama 6,99 pfu/ml, 7,82 pfu/ml ve 6,37 pfu/ml olarak tespit edildi.