Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 1999 / Cilt: 10 - Sayı: 1
Isolation or Paenibacillus larvae from the honey samples taken from the beehives and honey introduced for consumption
- Pages
- 93–100
- DOI
- —
Abstract
This study was performed for the detection of the prevalence of Paenibacillus larvae the causative agent of American Foulbrood Disease in the specimens collected from honey submitted to human consumption and also from healtl1y hieves in the various regions of Turkey. For this purpose. totally 327 honey specimens brought to The Antibiotic Disc Production Laboratory of the Central Veterinary Control and Research Institute were examined. The material were propagated initially in the nutrient agar and the vegetative forms were transferred to the broth of the same medium. Then. the honey specimens were dialized and cultivated in the brain hearth infussion agar and cultıvated in to the blood agar containing 6 mg/ml nalidixic acid. All the tests suggested for P. larvae were performed for obtaining the results. The agents were isolated from ı 4. ı 4 % of the 297 ho ney specimens that were collected from hieves and ı o% of the 30 honey specimens brought from the market.
Özet
Bu çalışmada Türkiye'nin değişik bölgelerindeki sağlıklı kovanlardan ve tü.ketime sunulmuş ballardan alınan örneklerde. Amerikan Yavru Çürüklüğü Hastalığı etkeni olan Paenibacillus larvae'nın yaygınlığı tespit edilmiştir. Bu amaçla Merkez Veteriner Kontrol ve Araştırma Enstitüsü Arıtibiyotik Disk Üretim Laboratuvarına getirtilen 327 bal numunesi incelenmiştir. Materyal öncelikli olarak nutrient agarda üretilmiş ve oluşturulan vegetatif formlar aynı mediurnun agarına transfer edilmiştir. Dal1a sonra bal örnekleri dialize edilerek brain hearth infussion agara ve 6 ,ug/ml nalidixic asit eldenmiş kanlı agara ekilmiştir. P. larvae için önerilen tüm testler uygulanaralt sonuca gidilmiştir. Kovanlardan toplanan 297 bal örneğinin %14.14'ünden, piyasadan satın alınan 30 bal örneğinin de %10'undan etken izole edilmiştir.