Transcriptional splice variants of CD40 and its prognostic value in breast cancer

Abstract

CD40 is an important tumor necrosis factor receptor (TNFR) family protein for the development of antitumor responseagainst cancer cells, apart from its role in the regulation of the immune system as a costimulatory molecule. It is broadly expressed onthe surface of immune cells and in diverse cancer types, including breast cancer. Here, we analyzed both CD40/CD40 ligand expressionin breast cancer cells and tissues using public data sets and overall survival analysis in ungrouped breast cancer patients, as well asin the triple-negative breast cancer subtype. We detected CD40 gene expression along with its 3 different splice variants (variants1–3), predominantly in the triple-negative subgroup of breast cancer cell lines. The results of the overall survival analysis showed thathigh CD40 gene expression, particularly in the triple-negative subgroup of breast cancer patients, is associated with better survival. Inaddition to the transcriptional levels of CD40 splice variants, investigation of protein levels of these variants will allow the categorizationof breast cancer cells and reveal their potential as an immunotherapeutic target.