Galektin-3’ün kadmiyuma bağlı kardiyotoksisiteye katılımı

Nalan AKYÜREK, Onur ERDEM, Cengiz GÜVEN, Nuray YAZIHAN, Ahmet SAYAL, Ethem AKÇIL, Mehtap KOÇAK KAÇAR

Abstract

Objective: Accumulation of the wide spread environmental toxin cadmium (Cd) in tissues results in toxicity. Heart is one of the most effected tissues. Cd exposure induces inflammation in effected tissues. The present study was focused to evaluate roles of tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) in Cd toxicity and their relationships with galectin-3 levels. Methods: In this experimental study, male Wistar rats were divided randomly to control and experimental groups. Experimental group was exposed to Cd at the dose of 15 ppm for 8 weeks (n=10/group). Inflammatory status in hearts was evaluated with measurement of tissue TNF-α and IL-6 levels. Histopathological examination of heart was carried out by light microscopy. Heart tissue caspase-3 level was used to identify apoptosis. Tissue galectin-3 level was evaluated by ELISA. Statistical difference between groups was evaluated by unpaired Student t-test, correlation was analyzed by Pearson’s test. Results: Heart sizes were increased after Cd toxicity. A significant increase in galectin-3 tissue levels was seen after Cd toxicity, this was accompanied with a significant increase in the TNF-α (control: 402±39, Cd: 793±26 pg/g tissue, p

Özet

Amaç: Çevresel toksinlerden biri olan kadmiyumun dokularda birikimi ciddi toksiteye neden olur. Kalp en sık etkilenen organlardan biridir. Kadmiyuma maruz kalma dokularda enflamasyonla sonuçlanır. Bu çalışmada kadmiyum toksitesinin kardiyak dokuda proenflamatuvar sitokinlerden tümör nekrozis faktör-alfa (TNF-α) ve interlökin-6 (IL-6) düzeylerine etkisi ve doku galektin-3 düzey değişiminin belirlenmesi hedeflenmiştir. Yöntemler: Bu deneysel çalışmada, erkek Wistar sıçanlar randomize kontrol ve deney grubuna ayrılmış, deney grubu 8 hafta, günlük 15 ppm kadmiyuma maruz bırakılmıştır (n=10/grup). Kalp dokusunda enflamasyon doku TNF-α ve IL-6 düzeyleri ile belirlenmiştir. Kardiyak doku histolojik olarak incelenmiştir. Dokuda apoptozis caspase-3 düzeyleri ile belirlenmiştir. Galektin-3 düzeyi ELISA ile ölçülmüştür. Gruplar arası farklar t-testi, korelasyon Pearson’s testi ile değerlendirilmiştir. Bulgular: Kadmiyum uygulanan grupta makroskopik olarak kardiyomegali gözlenmiştir. Dokuda TNF-α (kontrol: 402±39, Cd: 793±26 pg/g doku, p