Journals / Viral Hepatitis Journal / 2019 / Cilt: 25 - Sayı: 3
Evaluation of Signal/Cut-off Ratio by Anti-hepatitis Delta Virus Enzyme Immunoassay Method in the Diagnosis of Hepatitis Delta Virus Infection
- Journal
- Viral Hepatitis Journal
- Pages
- 97–100
- DOI
- —
Abstract
Objectives: The hepatitis delta virus (HDV) is an enveloped, negative-sense, single-stranded RNA virus in the genus Deltavirus. In this study, it was aimed to evaluate the ratio of anti-HDV signal/ cut-off (S/CO) in the diagnosis of HDV infection in patients who were positive for anti-HDV. Materials and Methods: Between August 2014 and December 2018, 156 patients, who were detected hepatitis B surface antigen (HBsAg) and anti-HDV positivity and were analyzed HDV-RNA, were included in the study. Anti-HDV antibody and HBsAg tests were analyzed by micro-ELISA method in serum samples and HDV-RNA was studied by real-time polymerase chain reaction method in plasma samples. Results: HDV-RNA was detected in 42.9% (67/156) of the anti-HDV positive patients. The mean S/CO value of anti-HDV in HDV-RNA positive group (8.99±3.53) was significantly higher than HDV-RNA negative group (5.99±3.73) (p
Özet
Amaç: Hepatit delta virüsü (HDV), Deltavirus genusunda, zarflı, negatif polariteli, tek iplikli bir RNA virüsüdür. Bu çalışmada, anti-HDV pozitif olan hastalarda HDV enfeksiyonu tanısında anti-HDV sinyal/ cut-off (S/CO) oranının değerlendirilmesi amaçlandı. Gereç ve Yöntemler: Ağustos 2014-Aralık 2018 tarihleri arasında hepatit B yüzey antijeni (HBsAg) ve anti-HDV pozitif saptanan ve HDV-RNA testi çalışılmış toplam 156 hasta çalışmaya dahil edildi. Anti-HDV antikoru ve HBsAg testleri serum örneklerinde mikroELISA yöntemi ile, HDV-RNA testi ise plazma örneklerinde real-time polimeraz zincir reaksiyonu yöntemi ile çalışıldı. Bulgular: Anti-HDV pozitif hastaların %42,9’unda (67/156) HDVRNA saptandı. HDV-RNA pozitif grubun anti-HDV S/CO ortalaması (8,99±3,53), HDV-RNA negatif gruba (5,99±3,73) göre anlamlı olarak yüksek saptandı (p