Investigating the effect of testosterone supplement on heart and muscle damage in rats applied with swimming exercise

Abstract

Anabolic Androgenic Steroids which are a synthetic derivative of testosterone are known to be used by some sportsmen inorder to improve performance. The present study was conducted to investigate the effect of testosterone supplement on heartand muscle damage in rats applied with swimming exercise. The study was conducted on 30 day-old 27 (Wistar, male) rats thatwere supplied from “Selçuk University Experimental Medicine Research and Application Center”. The rats were divided into 4groups. The rats in control groups (n:6) were fed during the study without doing any application. The rats in testosteronegroup(n:7) were supplied with a 10 mg/kg dose of testosterone 5 days a week for 3 weeks. The rats in exercise group (n:7) did freestyleswimming exercise for 30 minutes a day for 5 days a weekfor 3 weeks. The rats in testosterone + exercise group (n:7) didfreestyle swimming exercise for 30 minutes and were administered with 10 mg/kg dose of testosterone supplement for 5 days aweek for 3 weeks. At the end of the trial, necessary biochemical parameters were measured in serums obtained by drawingblood from tails of the rats. While levels of Serum, LDH, CK-MB, and CKwere studied by using an autoanalyzer, Troponin Ilevel was analyzed by using ELISA kit. SPSS 15.0 packaged software was usedin statistical evaluation of the data and the resultswere given as mean±SE. Since the data were convenient with the normal distribution, ANOVA test was applied forbetweengroups comparison and Duncan test was used to identify from which group the difference was resulted. Whenbetween-groups values of troponin I, ck-MB, and LDH, which are indicators of heart damage, and enzyme CK, which is anindicator of muscle damage,for control, exercise, testosterone, and testosterone+exercise groups were compared, a statisticallysignificant difference was determined between control, exercise, testosterone, and testosterone+exercise groups (p