Association of APOA5-1131T>C polymorphism with obesity in coronary artery disease

Aycan Fahri ERKAN, Berkay EKİCİ, Neslihan ÇOBAN, Maide KAŞİT, Nihan Erginel ÜNALTUNA, Aybike Sena ÖZUYNUK

Abstract

Objective: Genetic risk factors that cause coronary artery disease (CAD) demonstrate variations in different populations. In this study, a single nucleotide polymorphism in the APOA5 gene was targeted to determine genetic contributors to atherosclerotic CAD. The effects of this polymorphism on the development of CAD and known risk factors of the disease were examined. Methods: A total of 448 patients with angina or acute myocardial infarction who underwent coronary angiography were grouped as individuals with normal coronary arteries (≤30% stenosis) and critical disease (≥50% stenosis). The angiographic severity and the extent of atherosclerotic CAD were assessed using the Gensini and SYNTAX scores. Individuals were genotyped for the APOA5−1131T>C polymorphism using hydrolysis probes and the results were evaluated. Results: The APOA5−1131T>C polymorphism was associated with the serum lipid levels in the non-CAD group (pC and type 2 diabetes mellitus (p=0.055). This polymorphism was found to be associated with obesity and it was observed that the APOA5 -1131C allele carriers had a reduced risk for obesity (pC polymorphism was associated with important risk factors for CAD, obesity and serum lipid levels

Özet

Amaç: Koroner arter hastalığına (KAH) yol açan genetikrisk faktörleri toplumlar arasında farklılıklar göstermektedir.Aterosklerotik KAH oluşumuna neden olan genetik faktörlerin belirlenmesi amacıyla yapılan bu çalışmada, APOA5aday genindeki bir tek nükleotid polimorfiminin (SNP) KAHve KAH risk faktörleri üzerindeki etkisi araştırıldı.Yöntemler: Anjina veya akut miyokart enfarktüsü nedeniylekoroner anjiyograf yapılan 448 birey normal koroner arter(koroner lezyon ≤%30 darlık) ve anlamlı KAH (≥1 koronerlezyon ≥%50 darlık) taşımalarına göre iki gruba ayrıldı. Gensini ve SYNTAX skorları ile KAH’ın ciddiyeti ve yaygınlığı de-ğerlendirildi. Bireyler APOA5 −1131T>C polimorfimi için hidroliz probları kullanılarak genotiplendi ve sonuçlar incelendi.Bulgular: Çalışmamızda, anlamlı KAH olmayan grupta,seçilen APOA5 gen varyantı serum lipit düzeyleri ile ilişkilibulundu (p