Tavşanda L-NAME uygulamasının plazma, kalp dokusu NO ve MDA seviyelerine etkisi

Effect of L-NAME administration on plasma, heart tissue NO and MDA levels in rabbit

Abstract

Nitric oxide, produced in many mammalian tissues, is well known as an important mediator of many physiological and pathological responses. Nitric oxide synthesis plays an important role in many aspects of inflammation and wound healing. The purpose of this article is to comparatively investigate the effects of titanium, titanium + polyethylene glycol 4000, titanium + polyethylene glycol 4000 + N-nitro-L-arginine methyl ester and intraperitoneally injected N-nitro-L-arginine methyl ester, on plasma nitric oxide, malondialdehyde and, heart tissue nitric oxide and malondialdehyde levels. Plasma malondialdehyde, Nitrite and Nitrate and heart tissue malondialdehyde, nitrite and nitrate levels decreased after i.p. N-nitro-L-arginine methyl ester administration (p < 0.05). This leads to the conclusion that N-nitro-L-arginine methyl ester inhibits the deteriorating effects of free radicals without affecting wound healing. N-nitro-L-arginine methyl ester in polyethylene glycol 4000 and titanium also have no effect on tissue and plasma nitrite and nitrate and malondialdehyde levels meaning that nitric oxide generation is not affected from titanium and local nitric oxide synthase inhibitor.

Özet

Birçok memelinin dokularında üretilen nitrik oksit fizyolojik ve patolojik cevapların önemli aracısıdır. Nitrik oksit sentezi birçok inflamasyon durumunda ve yara iyileşmesinde önemli rol oynar. Bu çalışmada Titanyum, titanyum + Polietilen glikol 4000, titanyum + Polietilen glikol 4000 + N-nitro-L-arjinin metil ester ve intraperitonal olarak uygulanan N-nitro-L-arjinin metil esterin, plazma nitrik oksit, plazma malondialdehit, kalp dokusu nitrik oksit ve malondialdehit düzeylerine etkilerinin araştırılması amaçlanmıştır. Plazma malondialdehit, nitrit ve nitrat ve kalp dokusu malondialdehit, nitrit ve nitrat düzeyleri i.p. N-nitro-L-arjinin metil ester uygulaması ile azalmıştır (p