MCP-1 -2518 G ve rantes -403 A allelleri periodontitis için bir risk faktörü müdür?

Are monocyte chemoattractant protein-1-2518 G and regulated activation normal T Cell expressed and secreted -403 A alleles risk factor for periodontitis?

Abstract

Objective: We aimed to analyze the monocyte chemoattractant protein-1 (MCP-1) and regulated on activation, normal T cell expressed and secreted (RANTES) polymorphisms inrelation to susceptibility to chronic periodontitis and influence of genotypes in response tonon-surgical periodontal therapy. Methods: This cross-sectional clinical study was performed during 2005-2006 in Turkey.Gene polymorphisms were investigated in 51 periodontitis patients and 48 periodontallyhealthy subjects. Quadrant scaling and root planning was performed to periodontitis patients.Genomic DNA was analyzed for polymorphisms by polymerase chain reaction amplificationfollowed by restriction enzyme digestion. Results: Genotype distributions of related genes were similar between periodontitis andhealthy subjects (P>0.05). In periodontitis group, monocyte chemoattractant protein-1-2518G allele frequencies were 25.5%, regulated on activation, normal T cell expressed andsecreted-403A allele frequencies were 30.4%. Percentage of bleeding sites were significantlyhigher in regulated on activation, normal T cell expressed and secreted gene A negative genotypes compared to A positive (P=0.023). Significant improvements in clinical parameterswere found after periodontal therapy in both genotypes (P

Özet

Amaç: Bu çalışmada, “monocyte chemoattractant protein-1” (MCP-1) ve “regulated on activation, normal T cell expressed and secreted” (RANTES) gen polimorfizmlerinin kronikperiodontitise yatkınlık ile olan ilişkileri ve uygulanan cerrahisiz periodontal tedaviye yanıtagenotiplerin etkisini araştırmayı amaçladık. Yöntem: Bu kesitsel klinik çalışma 2005-2006 döneminde Türkiye’de gerçekleştirildi. Genpolimorfizmleri 51 periodontitisli ve 48 periodontal sağlıklı bireyde incelendi. Periodontitisli hastaların her yarım çenesine diş yüzeyi temizliği ve kök yüzeyi düzleştirmesi işlemleriyapıldı. Genomik DNA polimeraz zincir reaksiyonu amplifikasyonu sonrası restriksiyonenzim dijesyonu ile -403G/A ve -2518A/G polimorfizmlerinin analizleri yapıldı. Bulgular: Periodontitisli ve sağlıklı bireylerde her iki genin de genotip dağılımları benzerdir(P>0.05). Kronik periodontitis grubunun -2518G allel frekansı 25.5%, -403A allel frekansı30.4% dür. Sondalamada kanamalı bölgelerin oranı „regulated on activation, normal T cellexpressed and secreted” geninde A negative genotiplerde, A pozitif genotiplere göre anlamlıolarak yüksektir (P=0.023). Her iki genin genotiplerinde cerrahisiz periodontal tedavisonrasında klinik parametrelerde anlamlı düzelmeler bulunmuştur (P