A novel immobilization matrix for the biosensing of phenol: self assembled monolayers of calixarenes

Phenolün biyosensörle belirlenmesi için yeni bir immobilizasyon matriksi: kaliksarenlerin kendiliğinden oluşan tek tabakaları

Suna TİMUR, Hacer AZAK, Bilal DEMİR, Filiz TASCİ, Didem Ag SELECİ, Huseyin Bekir YİLDİZ, Dilek Odaci DEMİRKOL, Serkan SAYİN

Abstract

Aim: The development of calixarene based phenol biosensor.Methods: This study describes the application of a calixarene derivative, 5,17-diamino-25,27-bis(3-thiol1-oxypropane)-26,28-dihydroxycalix[4]arene (HS-CalixNH2) which has both amino and thiol functionalities, in the practical surface modifications for biomolecule binding. The structure of HS-Calix-NH2 allows easy interaction with Au surface and one-step biomolecule immobilization. Self-assembled monolayers (SAMs) of p-aminofunctionalized mercaptoalkylcalixarene (HS-Calix-NH2) were formed onto the Au electrode. Then, Laccase (Lac) enzyme was immobilized onto the modified surface by crosslinking with glutaraldehyde (GA). Resulted electrode (HS-Calix-NH2 /Lac) was used for the electrochemical analysis of phenolic compounds at − 50 mV. Results: The linearity was observed in the range of 0.1–100 μM and 1.0–100 μM for catechol and phenol, respectively. The potential use of the biosensor was investigated for phenol analysis in artificial samples which simulate the industrial waste water, which is highly acidic and composed of concentrated salt, without needing any sample pre-treatment step. Conclusion: The prepared Lac biosensor has a potential for rapid, selective and easy detection of phenolic contaminations in samples.

Özet

Amaç: Kaliksaren temelli fenol biyosensörlerinin gelistirilmesi.Metod: Bu calisma amino ve tiyol fonksiyonel gruplara sahip, biyomolekül baglanmasina olanak saglayan kaliksaren türevinin (5,17-diamino-25,27-bis(3-tiyol-1-oksipropan)26,28-dihidroksikaliks[4]aren (HS-Calix-NH2) uygulamasini tasvir etmektedir. HS-Calix-NH2 nin yapisi altin yüzey ile kolay etkilesime ve tek basamakli biyomolekül immobilizasyonuna izin vermektedir. Ilk olarak HS-CalixNH2 kullanilarak altin elektrod yüzeyinde kendiliginden olusan tek tabaka meydana getirildi. Daha sonra lakkaz enzimi kaliksaren modifiye altin yuzeye glutaraldehit ile capraz baglanarak immobilize edildi. Elde edilen (HSCalix-NH2/Lac) elektrod fenolik bilesiklerin - 50 mV da elektrokimyasal tayininde kullanildi.Bulgular: Katesol ve fenol icin lineer aralik sirasi ile 0.1-100 ?M ve 1.0-100 ?M olarak tayin edildi. HS-CalixNH/Lac biyosensörü kullanilarak yapay endüstriyel atik suyunu simule eden asidik örneklerde fenol tayini hic bir ön uygulama basamagina gerek duyulmadan gerceklestirildi.Sonuc: Hazirlanmis olan lakkaz biyosensörü fenol kontaminasyonlarinin hizli, secimli ve kolay tayininde kullanilma potansiyeline sahiptir