Morphological and molecular characterization and phylogenetic position of a new record, Tylenchorhynchus zeae , and some known species of Telotylenchidae Siddiqi, 1960 and Merliniidae Siddiqi, 1971 from Iran

Abstract

In order to identify the plant-parasitic nematodes associated withBerberis vulgaris ,Crocus sativus , andZiziphus zizyphus ,360 soil samples were collected from the rhizosphere of these plants in the South Khorasan Province of Iran during 2012 2014. Amongthe identified species,Tylenchorhynchus zeaeSethi and Swarup, 1968 of Telotylenchidae Siddiqi, 1960 is a new record from Iran andthe isolate is described and illustrated based on morphological, morphometric, and molecular characteristics. The phylogenetic treeinferred from partial sequences of the 28S rRNA (D2D3 segments) grouped the Iranian isolate with otherT. zeaesequences, forminga strongly supported clade (Bayesian posterior probability: 100%).T.z e a eis reported here for the first time onZiziphus zizyphusforIranian nematofauna. Additional morphological and molecular information is also provided for other species includingAmplimerlinius globigerus ,Merlinius brevidens ,Pratylenchoides alkani ,P. ritteri ,Scutylenchus rugosus ,S. tartuensis , andTrophur u s imparfromB.vulgaris ,C. sativus , andZ. zizyphusin Iran.