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Journals / Turkish Journal of Veterinary and Animal Sciences / 2002 / Cilt: 26 - Sayı: 2

Comparing the birth rates of microinjected hybrid mouse zygotes transferred into outbred and hybrit foster mothers

Mikroenjeksiyon uygulanmış hibrid fare zigotlarının outbred ve hibrid alıcılara transferleri sonrasında elde edilen doğum oranlarının karşılaştırılması

Haydar BAĞIŞ, Şakir SEKMEN, Hakan SAĞIRKAYA, Levent KESKİNTEPE, Hande ODAMAN

Pages
329–333
DOI
—

Abstract

The purpose of this study was to compare the birth rates after transfers of microinjected hybrid mouse zygotes into hybrid CB6F1 (C57BL/6J X BALB/c) and outbred CD-1 foster mothers. One or two hours after microinjection of a b -actin-Gfp gene construct into the male pronucleus, hybrid mouse zygotes were transferred into CB6F1 and CD-1 foster mothers. In addition, non-microinjected hybrid zygotes were transferred into foster mothers of both strains. The overall data demonstrate that outbred CD-1 foster mothers are a better choice for transfer of microinjected hybrid mouse embryos, and there was a statistical difference between the transfers of microinjected zygotes into CB6F1 and CD-1 foster mothers (P

Özet

The purpose of this study was to compare the birth rates after transfers of microinjected hybrid mouse zygotes into hybrid CB6F1 (C57BL/6J X BALB/c) and outbred CD-1 foster mothers. One or two hours after microinjection of a b -actin-Gfp gene construct into the male pronucleus, hybrid mouse zygotes were transferred into CB6F1 and CD-1 foster mothers. In addition, non-microinjected hybrid zygotes were transferred into foster mothers of both strains. The overall data demonstrate that outbred CD-1 foster mothers are a better choice for transfer of microinjected hybrid mouse embryos, and there was a statistical difference between the transfers of microinjected zygotes into CB6F1 and CD-1 foster mothers (P