Vibrio parahaemolyticus ATCC 17802 inactivation by using methylcellulose films containing encapsulated bacteriophages

Abstract

In the present study, it was aimed to inactivate Vibrio parahaemolyticus ATCC 17802, which is an important human pathogen,by making use of edible methylcellulose films containing encapsulated bacteriophages. For this purpose, the bacteriophages used asbiocontrol agents specific for V. parahaemolyticus ATCC 17802 were isolated from raw bonito fishes (Sarda sarda) and then purified,and finally the stabilities of isolated bacteriophages were determined. The pure bacteriophage filtrates were encapsulated with sodiumalginate by employing an extrusion method and the edible methylcellulose films were prepared. The bacteriophage stabilities of theprepared films were analyzed on the 0th, 4th, 7th, and 14th days of storage in a refrigerator (4 °C) under darkness and in illuminatedroom (22 °C) conditions. The methylcellulose films were determined to release bacteriophages to the environment and into raw fish filletsand antimicrobial effects of these films were detected against V. parahaemolyticus. In conclusion, a specific antimicrobial effect on V.parahaemolyticus ATCC 17802 was achieved by using edible methylcellulose films containing encapsulated bacteriophages. The resultsof microbial growth analysis showed an approximately 2.65 log difference in microbial levels between the control and the treatmentsamples. By maintaining the bacteriophage stability of prepared films, a new film that reinforces bacteriophage release was developed.