The comparing of homogenization methods for oxidative and antioxidative analyses of sperm

Abstract

Pretreatment of sperm for oxidative and antioxidative analysis has been shown to be different in studies. These differencescan affect the result of analyses. The aim of this study was to compare the analyses of sperm homogenization methods. The semenof four rams were used in the study. Pretreatment procedures included the rotary, bead, and ultrasonic homogenization methods.Oxidative and antioxidative status of sperm samples was assessed by measuring serum lipid hydroperoxide, total oxidant status, freesulfhydryl groups (SH total thiol), ceruloplasmin, and total antioxidant capacity levels. As a result, the homogenization methods beforeoxidative and antioxidative status analyses significantly affected the result (P < 0.05), and the ultrasonic homogenization method wasthe most effective. It is recommended to use the ultrasonic homogenization as a pretreatment method to determine the oxidative andantioxidative status analyses in sperm.