Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle

Abstract

Oxidative stress interferes with oviduct functions including oocyte maturation, capacitation, fertilization, and embryo andgamete transport. This study aimed to determine activity of the antioxidants glutathione peroxidase (GPX-I), superoxide dismutase(SOD), and catalase (CAT) in bovine oviduct epithelial cells (BOEC) isolated from the isthmus and ampulla of the oviduct at estral (n =7) and luteal phases (n = 7) of the estrous cycle. The antioxidant activity was measured at the primary, first, and second passages of thecell culture, and was characterized by cytokeratin expression. The GPX activity increased over the passages in samples of the ampullaand the isthmus of each sexual phase without statistical significance. The SOD activity remained steady through the cell passages inboth sexual phases. CAT activity at the primary culture was higher in the ampulla compared to the isthmus in both sexual phases witha significant difference for the estral phase (P < 0.05), and it decreased over the passages with no significant differences. In conclusion,the antioxidant enzyme activity profile of BOEC did not differ by region or sexual cycle except for that of CAT, which was higherin the ampulla. Further studies should focus on SOD, GPX, and CAT activity for the mechanism of BOEC adaptation to an in vitroenvironment.