Importance of 14-3-3eta, anti-CarP, and anti-Sa in the diagnosis of seronegative rheumatoid arthritis

Akgün YAMAN, Salih ÇETİNER, Filiz KİBAR, Yaşar SERTDEMİR, Reyhan BİLİCİ SALMAN, Emrah SALMAN, Suade Özlem BADAK, Emine Duygu ERSÖZLÜ, Alev ÇETİN DURAN, Barış BORAL, Eren ERKEN

Abstract

Background/aim: Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovial inflammation. The study aimed toassess serum 14-3-3eta, anti-CarP, and anti-Sa in seronegative RA (SNRA) patients who were treatment-naïve as well as in healthysubjects. This is the first study in the literature to examine these autoantibodies together in SNRA patients.Materials and methods: Forty-five treatment-naïve SNRA patients and 45 healthy subjects were recruited. Drugs change the levels ofautoantibodies; therefore, patients who took any medication had been excluded from our study. Anti-carbamylated protein, anti-Sa, and14-3-3eta were measured by using three different ELISA kits.Results: Median serum concentration of healthy controls in 14-3-3eta was 0.02 (0.02–0.27) ng/mL. Median serum concentration ofSNRA patients in 14-3-3eta was 1.00 (0.48–1.28) ng/mL. Data were analyzed with Mann–Whitney U tests; the P-value was