Does capsaicin have therapeutic benefits in human colon adenocarcinoma? Selection of the most reliable dose via AgNOR

Abstract

Background/aim: To determine the effect of different doses of capsaicin on AgNOR protein synthesis in human colon adenocarcinomaderivate from colon cancer (Caco-2 cell).Materials and methods: In this experimental study, after the cultured of Caco-2 cell line, the cells are divided into 4 groups as controland different capsaicin exposed doses (25uµ, 50uµ, and 75uµ). Mean AgNOR number and total AgNOR area/nuclear area (TAA/NA)were calculated.Results: A significant differences were detected between control and capsaicin (50uµ) (P = 0.001), between control and capsaicin (75uµ)(P = 0.000), between capsaicin (25uµ) and capsaicin (50uµ) (P = 0.001) and between capsaicin (25uµ) and capsaicin (75uµ) (P = 0.000)for TAA/NA. Also, there were significant differences between control and capsaicin (50uµ) (P = 0.001), between control and capsaicin(75uµ) (P = 0.000), between capsaicin (25uµ) and capsaicin (50uµ) (P = 0.000) and between capsaicin (25uµ) and capsaicin (75uµ) (P= 0.000) for mean AgNOR number.Conclusion: A certain amount of capsaicin has a protective effect against colon adenocarcinoma and the dose concentrations areimportant for the most reliable treatment.