An evaluation of the effects of caffeic acid phenethyl ester and Ankaferd blood stopper on secondary wound healing of oral mucosal tissue

Abstract

Background/aim: Caffeic acid phenethyl ester (CAPE) and Ankaferd Blood Stopper (ABS) are considered to contribute to woundhealing. The purpose of this study was to investigate the effect of ABS and CAPE on secondary wound healing of oral mucosal tissue.Materials and methods: In total, 63 male Sprague-Dawley rats were used in this study. The animals were randomly divided into threegroups and anaesthetized with ketamine (8 mg/100 g, intraperitoneally): a control group, CAPE group, and ABS group. A full-thicknessexcisional wound was created using a 4 mm punch biopsy tool. Topical ABS and CAPE were then applied in each group for 7, 14,and 21 days (n = 7 in each group). The animals in each group were sacrificed after 7, 14, and 21 days. Palatal specimens were stainedwith haematoxylin-eosin. Vascular endothelial growth factor (VEGF) and tumour necrosis factor-inducible gene 6 (TSG-6) proteinexpressions were determined using the Western blot method.Results: Inflammation, vessel dilatation, and haemorrhages were significantly lower in the CAPE group as compared with theseparameters in the other groups (P < 0.05). Fibrosis was significantly higher in the ABS group as compared with that in the other groups(P < 0.05). VEGF protein levels were elevated in the 21-day CAPE group and 7-day ABS group. The expression of TSG-6 increased inthe 7-day CAPE group and 21-day ABS group.