Baicalein Nanofiber Scaffold Containing Hyaluronic Acid and Polyvinyl Alcohol: Preparation and Evaluation

Abstract

Background/aim: Bone tumor is one of the major causes of tissue bone loss, particularly after performing surgical excision operationto bone lesion that needs to be replaced by biomaterials and ensure a complete filling of tissue-loss spaces. The purpose of our studywas to produce a nanofiber-based bone graft scaffold to fill the gaps resulted from bone cancer treatment and also capable of carryingfunctional molecules that can play a major role in preventing further cancer growth at the targeted bone tissue.Materials and methods: Electrospinning method was used in order to produce nanofibers from different kinds of polymers; Hyaluronicacid (HA), Polyethylene oxide (PEO) and Polyvinyl alcohol (PVA) blended with different concentrations of herbal antibiotic and anticancer flavonoid molecules called Baicalein (BE). The morphological and chemical structures of scaffold samples were studied usingScanning Electron Microscope (SEM), Fourier Transform Infrared-spectroscopy (FT-IR) and Surface-enhanced Raman spectroscopy(SERS) Analysis.Results: The results showed production of homogenous nanofibers-based scaffold (diameter between 80 nm and 470 nm) that containsthe polymers used in the spinning process and the entrapped Baicalein molecules within the nanofiber structure.Conclusion: It was concluded that successful formation of bone tissue mimicking scaffold can be achieved by using Electrospinningmethod that produces nonwoven nanofibers and at the same time can hold functional anticancer agent such as Baicalein, which mayallow using these types of scaffold in bone cancer treatment procedures.