Journals / Turkish Journal of Chemistry / 1999 / Cilt: 23 - Sayı: 3
Thin Layer Chromatographic Separation and Quantitation of L-Dopa and L-Tyrosine in Mixtures
- Journal
- Turkish Journal of Chemistry
- Pages
- 269–273
- DOI
- —
Abstract
A new TLC separation and quantitation method for L-tyrosine and L-Dopa mixtures was developed. The minimum tyrosine and Dopa quantities which can be measured by this technique are 0.7 and 1.5 m g respectively. The method may be used to measure the kinetic parameters of polyphenoloxidase as well as to trace the enzyme catalyzed conversion of L-tyrosine into L-Dopa. For a set of 20 measurements the maximum difference between any two measurements of spot areas was found to be 3 mm2 (4.5%) for L-Dopa (4 m g) and 2mm2 (5.6%) for L-tyrosine (1m g).
Özet
A new TLC separation and quantitation method for L-tyrosine and L-Dopa mixtures was developed. The minimum tyrosine and Dopa quantities which can be measured by this technique are 0.7 and 1.5 m g respectively. The method may be used to measure the kinetic parameters of polyphenoloxidase as well as to trace the enzyme catalyzed conversion of L-tyrosine into L-Dopa. For a set of 20 measurements the maximum difference between any two measurements of spot areas was found to be 3 mm2 (4.5%) for L-Dopa (4 m g) and 2mm2 (5.6%) for L-tyrosine (1m g).