Quantitative analysis of 1-hyoscyamine in Hyoscyamus reticulatus L. by GC-MS

Abstract

The hyoscyamine content of leaves and root samples of Hyoscyamus reticulatus} L. from Bulanık, Muş in east Anatolia, Turkey, was determined by capillary GC-MS to be 0.036$pm$ 0.004% in the leaves and 0.056$pm$ 0.011% in the roots as the predominant compound. Sopolamine was only detected in trace amounts. These findings are in accordance with reports on hyoscyamine content in Hyoscyamus} species. The limit of detection was 3.125 $mu$ g/mL and the limit of quantification was 6.25 $mu$ g/mL for hyoscyamine. This method has been shown to be linear and sensitive.