Determination of hydrogen peroxide with an enzymeless amperometric sensor based on poly(vinylferrocene)-supported Ag nanoparticles

Abstract

An enzymeless electrochemical sensor for detection of low amounts of H2 O2 with the aid of Ag nanoparticlessupported on conducting poly(vinylferrocene) (PVF) film was developed. Experimental results revealed that contributionof Ag nanoparticles led to remarkable improvement by means of reduction potential and reduction current. Influence ofexperimental parameters (i.e. polymeric film thickness, concentration of Ag precursor, immersion time in precursorsolution, reduction time, and reduction potential) were investigated. The Ag/PVF-modified electrode system wascharacterized physically by scanning electron microscopy. The results revealed that the sensor developed was easyto-prepare, economic, selective, and sensitive, with a fast response time of 3 s. The linear concentration range of thesensor was 0.1–50 mM, with a sensitivity of 14.1 µA mM −1and a limit of detection of 0.94 µM. Finally, interferenceeffects of uric acid, ascorbic acid, dopamine, and glucose molecules were studied and no significant interference wasobserved at physiological levels.