Histochemistry of glandular trichomes and the structure of selected organs of Borago officinalis L.

Abstract

In Poland, Borago officinalis is a cultivated plant or a segetal weed. It is a medicinal, cosmetic, melliferous, ornamental, andedible species. Its leaves, herb, flowers, and seeds are a source of bioactive compounds. Given the high concentration of essential fattyacids, borage is used in many branches of industry. The aim of this study was to examine the structure of trichomes and to carry outhistochemical labeling of selected groups of biologically active compounds contained in secretory cells. Anatomical traits of leaves,stems, and pedicels were investigated using bright-field light, fluorescence, and scanning electron microscopy. Characteristic features ofthe anatomical structure imply ecological adaptation of B. officinalis to xerothermic habitats. The trichomes in B. officinalis were dividedinto two groups. The first group included mechanical trichomes, whereas glandular trichomes formed the other group. Three hair typeswere distinguished in each group. Various groups of secondary metabolites were identified in exudates using histochemical assays. Theseinvestigations are a basis for identification of the location of selected groups of biologically active compounds such as essential oils, fattycompounds, neutral lipids, phenolic compounds, flavonoids, polysaccharides, and calcium in B. officinalis.