A magnetic affinity approach to identify plant GABA-binding proteins

Abstract

In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probingGABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA onmagnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magneticbeads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.