In vitro study on chondrogenic differentiation of human adipose-derived stem cells on treated bovine pericardium

Abstract

Bovine pericardium has been proposed as an available material for tissue engineering and bioprosthetic reconstruction. In thisstudy, bovine pericardium was fabricated into a scaffold for culturing and chondrogenic differentiation of human adipose-derived stemcells (hADSCs). Bovine pericardium was treated in 10 mM Tris-HCl and 0.15% SDS, followed by crosslinking in 0.1% glutaraldehyde.Treated bovine pericardium (tBP) was characterized as a slight yellowish thin membrane with enhanced tensile strength and strainproperty. The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBPas a cell-friendly scaffold for hADSCs due to low cytotoxicity and its ability to support an appropriate attachment and proliferation ofhADSCs. Moreover, there was an accumulation of the extracellular matrix proteoglycan in tBP seeded with hADSCs after 7 and 14days of chondrogenic induction. COMP as a specific marker of chondrogenesis was detected after 7 days, whereas type X-a1 collagen(Col10a1) expression was stable up to day 14. However, minor expression of aggrecan was found. Taken together, these results indicatethat tBP is a potential scaffold for hADSCs for cartilage tissue engineering.