Proximity mapping of the microtubule plus-end tracking protein SLAIN2 using the BioID approach

Abstract

The centrosome is the main microtubule-organizing center of animal cells, which plays key roles in critical cellular processesranging from cell division to cellular signaling. Accordingly, defects in the structure and function of centrosomes cause various humandiseases such as cancer and primary microcephaly. To elucidate the molecular defects underlying these diseases, the biogenesis andfunctions of the centrosomes have to be fully understood. An essential step towards addressing these questions is the identificationand functional dissection of the full repertoire of centrosome proteins. Here, we used high-resolution imaging and showed that themicrotubule plus-end tracking protein SLAIN2 localizes to the pericentriolar material at the proximal end of centrioles. To gain insightinto its cellular functions and mechanisms, we applied in vivo proximity-dependent biotin identification to SLAIN2 and generated itsproximity interaction map. Gene ontology analysis of the SLAIN2 interactome revealed extensive interactions with centriole duplication,ciliogenesis, and microtubule-associated proteins, including previously characterized and uncharacterized interactions. Collectively,our results define SLAIN2 as a component of pericentriolar material and provide an important resource for future studies aimed atelucidating SLAIN2 functions at the centrosome.