The Role of Metformin on Serum Vitamin B12 Levels in Patients with Type 2 Diabetes Mellitus

Abstract

Objectives. To evaluate the vitamin B12 (VB12) deficiency in patients with type 2 diabetes mellitus (DM-2) using metformin or other hypoglycemic agents. Methods. 400 patients with DM-2 were divided into two arms (N = 200/group); 1) those receiving metformin (MET) for at least six months and 2) those receiving hypoglycemic agents other than metformin (OHA). Serum VB12 concentrations were measured. Data were analyzed by using two-sample t-test for numerical data and chi-square with logistic regression analysis for categorical data. Numerical values were expressed as means ± standard deviations. Null hypotheses were rejected at P < 0.05. Results. Definite biochemical VB12 deficiency (