Dergiler / Tarım Bilimleri Dergisi / 2007 / Cilt: 13 - Sayı: 1

Bazı Ayva (Cydonia oblonga mill.) genotiplerinde yaprak disklerinden sürgün organogenesisi

Shoot organogenesis from leaf discs in some quince (Cydonia ablonga mill.) genotypes

Hatice DUMANOĞLU, Ahmet AYGÜN

Sayfa
54–61
DOI
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Özet

Geliş Tarihi: 06.11.2006

Abstract

Abstract: The objective of this study was to stimulate organogenesis of in vitro shoots from leaf discs in some quince (Cydonia oblonga Mill.) genotypes. Quince A (dwarf), S.Ö. (Sabahattin Özbek) 39-200 (dwarf), S.Ö. 18-82 (semi dwarf) and S.Ö. 58-315 (vigorous) clonal rootstocks, and Eşme, Limon, and Çukurgöbek cultivars were used as plant materials. Effects of TDZ + NAA combinations or BA + NAA, AgNO3 and putrescine treatments were investigated in two experiments for organogenesis. Shoot organogenesis was significantly induced on MS (Murashige and Skoog) basal medium containing 0.33 mg/l TDZ + 0.5mg/l NAA + 2.0 mg/l AgNO3. The rate of shoot formation was 80.0% in Quince A, 56.7% in S.Ö. 39-200, 17.5% in S.Ö. 18-82, 37.5% in S.Ö. 58-315, 36.7% in Eşme, 40.0% in Limon, and 43.3% in Çukurgöbek. The average shoot number per leaf disc was changed between 0.4 and 2.7 among genotypes.