Onkogenik agrobacterium tumefaciens a281 hattı ile çivit otu (ısatis constricta davis) bitkisinde tümör olusumu

Abstract

Abstract: Leaf and petiole explants obtained from in vitro grown seedlings of Isatis constricta (woad) were treated with oncogenic A281 strain of Agrobacterium tumefaciens. Tumour formation started on explants after 15 days and continued until 4 weeks when gus test was performed. The highest gus positive results were recorded from petiole explants selected on medium containing 50 mg/l kanamycine under 16 h light photoperiod.