Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

Abstract

This research was conducted at the breeding station of the Turkish breeding company Agromar A.Ş in the city of Bursain Turkey during the 2013 and 2014 growing seasons. Used from within the same heterotic group crossings, 7 donormaterials were obtained during the 2012 winter season in the greenhouse. The inducer line RWK-76xRWS, providedby University of Hohenheim, Germany, was used for generating haploid seeds. The donor and inducer crossing wasperformed during the 2013 summer season. The haploid selection and chromosome doubling were performed duringthe 2014 summer season. Seven donors were used for haploid induction which name are DNR1, DNR2, DNR3,DNR4, DNR5, DNR6, DNR7 respectively, from each donor diffrent amount of ear crosses were performed (DNR1:16ears, DNR2:10 ears, DNR3:10 ears, DNR4:12 ears, DNR5:11 ears, DNR6:13 ears, DNR7:11 ears). According to thepresent study, the average induction rate found ranged from 7.1 to 12.8%, and the average seedling survival rate in thegreenhouse after colchicine application ranged from 57.9 to 77.6%. After transplanting to the fild, 78.3-92.6% of theseplants survived. As a result of this research, the chromosome doubling rate ranged from 22.5 to 48.3% depending onthe donor material. These result indicates that maternal haploid selection visually is easy. Haploid induction rate (HIR)changes from donor to donor, its mean genotype and environment is effctive for HIR. Average chromosome doublingrate is lower than other researchers’ results, it is also effcted by genotype and chromosome doubling methods.